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I found article in Nature: A helper phage to improve single-chain antibody presentation in phage display Experimental protocol Standard cloning procedures, determination of colony-forming units and plaque-forming units, and immunoblot after PAGE were carried out according to Sambrook et al. Construction of the packaging cell line. ...


The fastest will change as time passes and better technologies are developed. I think the fastest method existing at the moment is Shotgun Mutagenesis (provided by Integral Molecular Inc). This does not employ any new method of doing that. They just provide a set of plasmids, that has all the possible mutations. The set itself is generated by automated ...

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