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I found article in Nature: A helper phage to improve single-chain antibody presentation in phage display Experimental protocol Standard cloning procedures, determination of colony-forming units and plaque-forming units, and immunoblot after PAGE were carried out according to Sambrook et al. Construction of the packaging cell line. ...


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Ants, slime molds, and brains. Ants and slime molds use simple rules to generate pretty good transportation networks in an emergent way, and brains wire and rewire themselves constantly(adding/removing edges, but not usually nodes). Evolutionary networks, metabolic networks, and ecological networks are much harder to get concrete data sets from, because ...



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