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I found article in Nature: A helper phage to improve single-chain antibody presentation in phage display Experimental protocol Standard cloning procedures, determination of colony-forming units and plaque-forming units, and immunoblot after PAGE were carried out according to Sambrook et al. Construction of the packaging cell line. ...


Ants, slime molds, and brains. Ants and slime molds use simple rules to generate pretty good transportation networks in an emergent way, and brains wire and rewire themselves constantly(adding/removing edges, but not usually nodes). Evolutionary networks, metabolic networks, and ecological networks are much harder to get concrete data sets from, because ...

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