New answers tagged

0

The best reference I could find is a book chapter (available here as a .pdf) from a book on optimizing the expression of membrane proteins that argues for T7. Although they don't appear to reference a source for that specific claim, some of the references close by could be helpful. It seems as though this applies in general, not just to membrane proteins. ...


0

Yes. In principle you will have a vastly increased ability to customize the boundaries of each protein domain with a primer and PCR based approach. It is not obvious to me that the Gateway Cloning System is required, or necessarily the best choice for this approach, but that is likely subjective. Are you familiar with, or aware of, the efforts of the SGC ...



Top 50 recent answers are included