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If you're expressing the gene in E. coli, then you're almost certain to be using a plasmid or other ectopic gene vector. There are a wide variety of plasmids available, with many different promoters. With a commercial plasmid, you're not adding in the promoter sequence, but the gene sequence. You pick the promoter you want and then clone your gene sequence ...


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The exact numbers depend on the protein. In my hands, I would say between the pAraBAD and a pLac-T5 you can expect some fold improvement and pAraBAD and a pT7 promoters you can expect about an order of magnitude… but there are two big caveats. First, pET and other T7 driven plasmids do not work in K-12 strains (without the pTARA) so BL21 is generally used. ...


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In general, T7 is stronger in producing mRNA. However, the final amount of protein produced depends on other factors like protein solubility, stability, and toxicity. Here, https://microbialcellfactories.biomedcentral.com/articles/10.1186/1475-2859-12-26, is a study that compares T7 and other promoters for the production of few different proteins.



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