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Every amino acid has a different isoelectric point: a pH where they do not carry electric charge. This isoelectric point depends on the side chains: By glycine the side chain $-H$ is neutral (while the amino and carbonic acid groups are not) so the IEP is 5.97. By lysine the side chain $-(CH_2)_4\mbox{-}NH_2$ is alkaline $[R\mbox-NH_2 + H^+ ...

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