Hot answers tagged proteins
10
Yes. All proteins actually begin to get synthesized on cytoplasmic ribosomes but if they are going to be used for extracellular purposes, they are tagged and whole ribosome is taken to ER where protein synthesis is completed. The proteins are exocytosed with help of Golgi body, the post office tagging and packaging organelle (the Golgi body packages these ...
6
The rationale for the choice of higher organisms as the producing source is based on costs and biological activity.
Biological activity. In their active forms, various proteins have
post-translational modifications (i.e. glycosylation) which are
difficult to reproduce in bacteria. Alan's answer is already
exhaustive.
Costs. Mammalian cell lines are easier ...
2
Like any other protein, antibodies will aspecifically bind nitrocellulose or PVDF membranes, but any other protein present in your antibody preparation will also do.
Depending on the antibody class, more specific binding can be obtained with protein A or protein G, that recognize the Fc domain. It's usual to have protein A/G immobilized on a stationary ...
1
I would like to complement dd3's answer a bit. It is about bacteria
Bacteria secret enzymes outside. The bacterial membrane has import size limits and it is necessary for them to chop the big molecule into smaller pieces before import. Examples are glycoside hydrolases and protease. Bacteria take part in the clean-up after the annual algal and jellyfish ...
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