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With a few exceptions among some bacteria, all species on the planet make protein from the same 21 amino acids, and the relative abundances of amino acids is very similar in proteins from plants, animals, fungi and even prokaryotes. See for example this article (available as PDF here). So protein from pretty much any food provides the same amino acids. The ...


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It's because E.coli BL21(DE3) are competent cells. The competent is the key here as the cells were chemically treated so the transfection can be performed by heat-shock with high efficiency. This means these bacteria are quite fragile, due to the chemical treatment, and therefore are very sensitive to both mechanical and thermal shocks. Pre-heating the ...


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Regular expressions are not commonly used for DNA or Protein analysis, but many people use Hidden-Markov-Models (HMMs). If you are looking at protein domains, you can find many HMMs here: http://pfam.xfam.org/


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Cooking is just a form of digestion. What is digestion? Digestion is the process of breaking down big molecules into smaller molecules. When you cook food you break down big molecules into its small components. Why do we digest food? Think about a long sequence of DNA for example. You eat corn and you have in your body a long sequence of corn ...


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Given your background (not a biologist or chemist) you would probably find the introductory material in a biochemistry "lite" textbook more accessible/useful than a hardcore text for specialists. As a co-author of a biochemistry textbook, I can tell you that there are essentially three different classes, or types of texts. Comprehensive textbooks, of ...


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Although quantitative methods using MS have been developed, MS is not inherently quantitative. Quantification with MS could be quite tricky. Therefore, it is not the first choice. But, if you do not know which protein levels change and want to find proteins the expression levels of which are different between your samples you are going to compare, MS is not ...


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Prosite (http://prosite.expasy.org/) uses regular expressions to search for protein domains, in contrast with Pfam. If you look at an entry, such as http://prosite.expasy.org/cgi-bin/prosite/prosite-search-ac?PDOC00022, you can see the consensus pattern under the PATTERN section towards the bottom. Prosite does not have the same coverage as Pfam, but the ...


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Your question is answered in the paper, the protein likely exists as a homodimer in vivo and denaturation (such as performed with SDS PAGE -> Western Blot) separates the dimers into monomers: "To determine the quaternary structure, size exclusion high-performance liquid chromatography was performed using an Agilent 1100 series high-performance liquid ...


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Each daughter cell, following mitosis, will contain some ribosomes and DNA transcription machinery already, so it can already synthesise more ribosomes. In the case of the fertilised ovum, when the organism is only a single cell, the first ribosomes will be those already present in the unfertilised ovum. So cells never have to start from scratch with no ...


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From the comment section: Cells divide and when they do the two daughter cells will not only have copies of the DNA but also of the organelles, the cellular machineries and the cell membrane which implies that yes the daughter cells receive also ribosomes. The replication happens during the interphase.



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