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There are not much explanations available, as far as I can see. The best explanation that I have found is that the positive charge allows the orientation of the protein in the membrane. The orientation for membrane proteins is important as a lot of them are transporters which have a dedicated transportation direction. The same is true for receptors, which ...


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Rosetta strain is normally used when people try to expression mammalian genes in E. coli. Origami™ strain is used for protein folding. you may need triple antibiotics to select your clones. This may impede bacterial growth.


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6kDa are usually enough to seperate two proteins in my experience. Try the other way, use a 7 or even 8% gel. They run longer but have a higher separation capacity. If you look at this figure from the LabFaQ, you see that you can use a higher percentage gel: The other thing are the antibodies: Where are your isoforms different? 6kDa are 55 to 60 amino ...


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γ-Tubulin is specifically localized to the minus end of microtubules. GFP labeled γ-Tubulin can be used to label the minus ends in-vivo and without the requirement of staining. Fan et al have developed a phage display antibody specific to α-Tubulin that can be used to visualize minus ends. See the figure below. ...


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This an initial solution, but can be improved: out = system2("f:/stride/stride.exe", "f:/stride/1a11.pdb -h", stdout=T) nh = strsplit(out[grepl("HBT", out)], " ")[[1]][2] print(nh) # Output: 26 (hydrogen bonds) I compiled an Windows version of Stride and uploaded here: http://lcrserver.icmc.usp.br/~daniel/bin/stride.exe


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This is a general answer for all three of your related questions: This one How does Temperature influences the rate of protein turnover? How is the rate of transcription influenced by temperature? Since you said: I want to simulate the evolution of genetic architecture when after a sudden change in temperature or in an environment that is ...


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The number of hydrogen bonds cannot actually be said from software only inferred. But you could try using STRIDE (http://structure.usc.edu/stride/) which takes the file name with a -h flag to output the number of hydrogen bonds. You could then write a small shell script which would pass in each file and store the data you wanted in whatever format you liked. ...



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