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I am not sure about what they used in 1958 (perhaps Sorvall). However you may look at recent papers for ECM protein isolation (if that was your objective). Have a look at this article. From materials and methods: ECM protein isolation. Fibroblasts or A431 cells were removed from the surface of the culture plate with 2 mM EDTA in PBS for 3–5 min ...


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This is too long for a comment, so I post my thoughts about this here: This falls into different important parts here: One is an insufficient understanding of the techniques on which the method is based. This makes it hard to decide which steps are important and which are not. One prominent example would be the use of EDTA in DNA gel running buffers. This ...



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