New answers tagged purification
It works pretty well and can be used to desalt DNA. The DNA runs a bit different than proteins since it is more a long stretched molecule while a lot of proteins are globular. See this references (the first is about the behaviour of DNA on superose): Size-exclusion chromatography of DNA restriction fragments. Fragment length determinations and a comparison ...
The pI (iso-electric point) is the pH at which the protein (or other molecule), overall has a net zero charge. As @Chris points out, the buffer you are using will change the pH the protein finds itself in. This will change the charge of the protein. In ion exchange chromatography, there are charged chemical groups on the resin and these can cause a ...
Have a look at this table, it really depends on the buffers you use: The table is from this website, which is definitely worth reading when you are interested (or work) with ion exchange chromatography.
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