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I remember going to a chemistry lecture which mentioned this (the lecture was on blood transfusion in general). The main reasons are: 1) Hemoglobin is toxic to the human body: Hb in RBCs is a tetramer, but in the plasma it breaks into two dimers --> toxicity in the kidneys. Hb needs modification by cross-linking or recombination. Wikipedia states that this ...


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Yes it can. You will want to use a thin hemocytometer and the working distance of the objective (20x) needs to be long enough to focus both the cells under the cover glass and the grid under the hemocytometer. Because you are looking at it from the top it would be helpful probably to have some phase on the scope to increase your contrast so that you can more ...



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