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A similar question was already answered at How do Gram + bacteria use a proton gradient for F-type ATPase? . In those answers, there is a general belief that Gram negative bacteria do not have much control over their intermembrane space pH, because their outer membranes have many porins, such as OmpC, which allows free passage both ways for protons and other ...


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In a strict sense, I think you are correct that [H$^+$] contributes directly to the Bohr effect. A paper by Perutz et al. (1980) identified at least two amino acid residues in hemoglobin that account for the Bohr effect by interacting directly with H$^+$. CO$_2$ is not directly involved. Further, the Bohr effect (and the Root effect) are defined in terms of ...


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Would I be correct in saying that the Bohr effect is ONLY related to the concentration of [H+] in the tissues. I would say no. Because oxygen binding affinity is inversely related to both [1]: high $[H^+]$ concentration and $CO_2$ increase (which is can be a consequence of the first) $CO_2$ is involved in the Bohr effect: The biological ...



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