Involves library preparation and sequencing. Library preparation might slightly vary for different classes of RNAs. General procedure for mRNA and most ncRNA sequencing involves:
- Enrichment of polyA RNAs using oligo-dT beads to remove ribosomal RNA. Alternatively, ribosomal RNAs can be selectively removed. Refer this.
- cDNA preparation
- Adapter ligation
- PCR amplification
- Sequencing
