Involves library preparation and sequencing. Library preparation might slightly vary for different classes of RNAs. The general procedure for mRNA and most ncRNA sequencing involves:

  1. Enrichment of polyA RNAs using oligo-dT beads to remove ribosomal RNA. Alternatively, ribosomal RNAs can be selectively removed. Refer to this.
  2. cDNA preparation
  3. Adapter ligation
  4. PCR amplification
  5. Sequencing

See also .

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