Tag Info

New answers tagged

2

The process of sequencing was, and can be, assisted by cloning a DNA fragment into a known site in a plasmid designed to help sequencing. That cloning site is flanked by a known sequence(s) that can use standard primers In the figure "A" and "B" are just reference points on either side of the Cloning site. Of course, the plasmid extends on either side of ...


6

The MIT synthetic chemist Gobind Khorana won the 1968 Nobel Prize in Chemistry for his work which successfully was able to make chains of Ribonucleic acids. The chemistry was difficult at the time but he won the prize for making specific sequences of RNA bases which were then fed to cells, resulting in specific amino acid chains, which ultimately deciphered ...


2

For the classic Sanger sequencing you need quite large amounts of DNA to be able to run the sequence reaction. This is a problem when you want to sequence whole genome since the DNA needs to be amplified then, which for some sequences can cause problems, introduce errors or cause bias. Single molecule sequencing is a huge step forward here, since only one ...


1

blast2go is on the verge of getting commercialized (as they have started selling PRO versions) and my previous experience was not so good with it. I used IntrProScan to associate GO terms with the transcripts, it ran long but it reported all the possible sequence features. To use these custom annotation was tricky for visualization, but thanks to BiNGO, I ...



Top 50 recent answers are included