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Have you discovered wormbase yet? It will become your new best friend. In worms gene names reflect the loss-of-function (lf) phenotype. Daf means "abnormal DAuer Formation," so daf-2 was the second daf gene identified (ref). The mutations are called alleles and each worm lab has their own allele designation. e was for England and in that lab's strain list ...


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Okay, first, I would say to everyone planning a high-throughput experiment: Hypothesis first, then experiment. Otherwise you're setting up an expensive and time-consuming test that's not going to answer the questions you want answered. In this case, the question your experiment is designed to answer is "What genes are crucial to growth in vivo that are not ...


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To find out whether a dataset was paired-end or single-end, go to SRA, click on a run, and look under "Library". Paired-end datasets will typically have "Layout: paired". Note that people don't always mark this correctly, which causes no end of headaches. Regarding lines like "CCCFFFFFGFHHHGJJJJI#1?FEIGGI", that's the quality score line. Look at the fastq ...


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Go to NCBI-SRA or GEO and search for RNAseq data. Set organism as C. elegans and optionally any other keywords that you are interested in. This search term will give you all RNAseq data in SRA from C. elegans: ("caenorhabditis elegans"[Organism]) AND "strategy rna seq"[Properties] You will generally find files in fastq format which you can easily convert ...



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