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I think given that you're just getting started with genetics, you can say that the codons are interchangeable. This is generally true, though not technically correct. A few reasons immediately come to mind for why this is the case: As pointed out in the comments, specific organisms use specific codons with different frequencies. This is usually related to ...


There can be several reasons for variability and since you said that the machine has not been serviced for a long time, I guess that should be one main source of errors. Multichannel pipettes can also sometimes cause problems (based on personal experience) and it is mostly because the tips don't fit properly in all channels. Try varying some parameter (for ...

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