Hot answers tagged rna
Knockdown of lncRNA in mammals is not done via RNAi. Instead, one transfers antisense DNA oligos which bind to the RNA. This triggers the action of the RNase H enzyme, which degrades RNA-DNA duplexes. It degrades the lncRNA. UPDATE: For reference, I learned about this from a seminar, and it is not very well documented, but after some literature search I ...
Nuclear RNAi happens.. check these articles: http://www.nature.com/nrg/journal/v14/n2/execsumm/nrg3355.html http://nar.oxfordjournals.org/content/early/2011/11/07/nar.gkr891.full Also there is some evidence that Ago2 binds to lncRNAs. However, you can employ other techniques to knock down lncRNAs for e.g. ribozymes.
The Ambion's RNAseZAP is not a detergent but contains chemicals that denature RNAse, the step of rinsing with water is necessary in order to clean up the chemicals as well as the denatured proteins left. It is highly recommended as it completely wipes out the RNAse and the experiment can be run safely. No need to risk time, money and samples. So, to sum up, ...
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