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This paper uses a similar technique to you by purifying total RNA, synthesis of double-stranded cDNA containing a T7 promoter sequence, cDNA purification, in vitro transcription in the presence of biotin-labeled ribonucleotides and this transcription is possible with T7 RNA polymerase since the cDNA contains the T7 promoter region, and finally fractionation ...


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Having looked into this question, I came across this paper, where they mixed biotin labelled EGFR-mutant-specific oligonucleotide and isolated the target using streptavidin beads followed by real-time TaqMan PCR allowing a 40 fold increase in detection sensitivity of the mutant allele, suggesting that biotin does not effect nucleotides in PCR based ...


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RNA was almost certainly the first genetic molecule of inheritance. However, the single-stranded nature of RNA is not particularly stable and thus would not be reliable for the long-term storage of genetic information necessary for reproduction (and ultimately evolution). The necessary stability is provided by DNA. The question becomes, how did DNA evolve ...


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Imagine you want to produce a widget. You have thousands of worker, but only one blueprint. Each worker needs the blueprint to build a widget (they're really forgetful and can't build from memory). So only one worker at a time can build your widgets. What you would do is to create copies of your blueprint and distribute them to your workers. That way ...


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OK, I found this on Wikipedia. I probably should have checked there first! DNA has three primary attributes that allow it to be far better than RNA at encoding genetic information. First, it is normally double-stranded, so that there are a minimum of two copies of the information encoding each gene in every cell. Second, DNA has a much greater ...


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Yes, that should be possible. And it is one of the ways antibodies work. It is already used as a treatment against rabies. There you get a dose of immunoglobulins directed against the rabies virus together with the vaccine. The immunoglobulins neutralize the virus. The same is possible when you vaccinate against the surface proteins which a virus uses to ...



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