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10

I agree with @Jeremias Brand's answer. Pretty much you will have to forget about fluorescence microscopy... you can probably find some dusty old one on eBay in your price range, but it probably won't be any good. However, the good news is that seen that in your comment you mention a) plants, b) blood, c) liquids such as wine, d) food? transmitted ...


8

With all respect I think the accepted answer underestimates the quality of current inexpensive instruments. What I have found comparing images on my recently acquired $400 scope to those produced by top-end Nikons is that it produces images which are aesthetically less appealing but nearly identical in detail. Mostly I have used it for fungi, which are ...


7

Wear two sets of gloves. First, use nitrile gloves, not latex for this. 4mm or thicker is a specification I would recommend to avoid tearing. Latex will bind too well to itself and tear easily with increased friction etc (same logic behind not using two condoms). Also, use a coat with an elastic cuff on the sleeve. So here's the breakdown Put on first ...


6

The water from taps labelled "Industrial Water, Do Not Drink" comes from the same potable water line as domestic taps. The line should be split somewhere in the building, with the industrial line containing multiple backflow inhibitors to prevent cross contamination of potable or domestic water. Based on that fact, the water should be safe for hand washing, ...


6

This really depends on the application you have in mind. As with other precision instruments there is a huge range of qualities and applications. If you just want brigth field illumination and look at relatively big things ( approx 100 microns) then you could find something decent for the price you mention if you buy used. But if you want more complex ...


4

A neutralization reaction occurs between Brönsted acids and bases to form a salt. In this case, you have to add a Brönsted base to neutralize your acid. If the final pH is important, you should work out the exact quantity of base to add. Otherwise, just use litmus paper to check the pH of your solution, and add base until it's neutral. EDIT In view of the ...


4

This is kind of a weird/trick question. How long do you want the animal to live? If the lifespan is shortened or compromised does that fit.... Obligate carnivores (cats, dogs) do eat plant material. In the wild cats mainly eat grass to get rid of hairballs. Cats are more impacted by phytotoxins than dogs. However, both, are extremely vulnerable to ...


4

I usually find the treatment of Ethidium bromide in the lab widely exaggerates the true dangers of this chemical. It is not really toxic (the LD50 is 1.5 g/kg bodyweight, as a comparision NaCl has a LD50 of 3 g/kg). It has also only been shown to exhibit mutagenic potential to bacteria after it had been treated with liver extracts. This means that some of ...


3

Autoclaving is the best way for sterilization. If your growth medium is large, how much chemical disinfectant do you think you would need to bring up to its working concentration? Add some acid to your waste medium (so that it's only weakly acidic), not as disinfectant, but to suppress ammonia formation. Urea will still break down during autoclave, but ...


3

Inspired by Jason Patterson's comment, I looked up "ethidium bromide cattle" and found a blog post about ethidium bromide toxicity. Apparently ethidium is routinely used to treat sleeping sickness in african cattle at 1mg/kg without ill effects. All data concerning ethidium toxicity comes from in vitro tests that may not apply to in vivo use. You should ...


3

First we will take a look at the lines of resistance of a carrot. There are three The skin - The first line of resistance is the outer membrane or skin of the carrot and chemical compounds that are normally present there. Two antifungal polyacetylenes, falcarinol and falcarindiol, and an isocoumarin, 6-methoxymellein, are present in small amounts in ...


2

Very popular microscopes for bio work these days are the inverted style, These allow you to view samples without preparing slides. http://en.wikipedia.org/wiki/Inverted_microscope http://www.biotechequipmentsales.com/equipment-for-sale/details/564/14/microscopes/nikon-eclipse-ts100


2

I think that the distinction between carnivores and herbivores contains a gray area with a spectrum of omnivores in-between. Bears are placed in the order of carnivores, but are definitely truly omnivores see link. However, if you give them a choice between a salmon and blueberries, it's quite clear what will be devoured first. That's why it's a carnivore. ...


2

Work with vectors expressing oncogenes or knocking down tumor suppressor genes (particularly if vectors are based on lentivirus) is often done under "BSL-2+" conditions. This essentially means that personal protective measures required for BSL-3 are used, but the specialized ventilation systems and so forth of BSL-3 physical laboratory space are not ...


2

Maybe, cells should be contained under BSL-2 and agents (viral particles etc) under stricter BSL-3? That is, after immortalization you can keep cells under lower security. Biosafety Level 2 builds upon BSL-1. BSL-2 is suitable for work involving agents that pose moderate hazards to personnel and the environment. Biosafety Level 3 is applicable to ...


1

Your eyes can dilate behind sunglasses, especially when the main source of light (the sun) is blocked, and ambient light decreases. Regular sunglasses offer some protection against certain UV wavelengths, but not enough to protect your eyes when looking directly at the sun (or, during an eclipse, the corona). Most sunglasses are mainly designed to, first and ...



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