Tag Info

New answers tagged

2

Mouth pipetting, while almost unheard of in modern laboratories in developed countries, is still very much a current protocol in many parts of the world. For example, this paper analyses the proportion of clinical labs in Pakistan and found evidence of poor biosafety practices (emphasis mine): Results: A total of 1,647 (92.4%) males and 135 (7.6%) ...


2

Work with vectors expressing oncogenes or knocking down tumor suppressor genes (particularly if vectors are based on lentivirus) is often done under "BSL-2+" conditions. This essentially means that personal protective measures required for BSL-3 are used, but the specialized ventilation systems and so forth of BSL-3 physical laboratory space are not ...


2

Maybe, cells should be contained under BSL-2 and agents (viral particles etc) under stricter BSL-3? That is, after immortalization you can keep cells under lower security. Biosafety Level 2 builds upon BSL-1. BSL-2 is suitable for work involving agents that pose moderate hazards to personnel and the environment. Biosafety Level 3 is applicable to ...



Top 50 recent answers are included