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I usually find the treatment of Ethidium bromide in the lab widely exaggerates the true dangers of this chemical. It is not really toxic (the LD50 is 1.5 g/kg bodyweight, as a comparision NaCl has a LD50 of 3 g/kg). It has also only been shown to exhibit mutagenic potential to bacteria after it had been treated with liver extracts. This means that some of ...


3

Inspired by Jason Patterson's comment, I looked up "ethidium bromide cattle" and found a blog post about ethidium bromide toxicity. Apparently ethidium is routinely used to treat sleeping sickness in african cattle at 1mg/kg without ill effects. All data concerning ethidium toxicity comes from in vitro tests that may not apply to in vivo use. You should ...


7

Wear two sets of gloves. First, use nitrile gloves, not latex for this. 4mm or thicker is a specification I would recommend to avoid tearing. Latex will bind too well to itself and tear easily with increased friction etc (same logic behind not using two condoms). Also, use a coat with an elastic cuff on the sleeve. So here's the breakdown Put on first ...



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