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An SDS-PAGE standard curve of Rf vs LogMW is linear because the standard you're given is engineered to behave that way. An expanded SDS-PAGE curve would show that the actual graph is sigmoidal: Structues that are too large can't enter the matrix, and too small they pass right through. Your standard has taken into account the % composing the gel, and the size ...


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Short Answer - If the same antibody works both for IHC and SDS-PAGE it either means it is monoclonal against a surface linear epitope or a polyclonal antibody. Long Answer - Starting off, there are broadly two kinds of antibodies based on the type of the epitopes. The epitope can either be linear or conformational. Here's a couple of pictures that should ...


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Many but not all B cell epitopes will be destroyed by denaturing. Those that are conformational will be destroyed, but there are also many B cell epitopes that are linear, based on amino acid sequence only without strong conformation dependence. Also, depending on your IHC protocol, there's often a fixation step that involves a certain amount of ...



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