New answers tagged sequence-analysis
I think your supervisor wants to see if there is inter-cellular variation in the repeat length and if so, calculate the variation. This may be compared with inter-tissue or inter-individual variation. Usually when you take a pool of cells for any assay, you would average out the properties of individual cells. Using sequencing you may actually be able to ...
Using the SeqRecord object approach with Bio.SeqIO, you would do something like this: my_record.annotations["data_file_division"] = "PLN" my_record.annotations["date"] = "24-DEC-2015" Note that only valid NCBI GenBank divisions, or their EMBL equivalent, will be accepted.
Unfortunately, it does depend on sequencing techniques. For example, in Illumina sequencing, each sequence fragment is amplified (in order to get a stronger signal) and forms a cluster on the microarray. Each cluster is sequenced by cycles of: Adding fluorescent terminator nucleotides. These nucleotides are modified to contain an inhibiting/terminating ...
This is the best review I have seen with a comprehensive discussion on gene duplication and amplification mechanisms (and it is also quite recent). It seems like there may be no definitive answers to your question in the literature--perhaps because of a paucity of viable experimental models that would allow one to test various hypotheses. Also @canadianer ...
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