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I think your supervisor wants to see if there is inter-cellular variation in the repeat length and if so, calculate the variation. This may be compared with inter-tissue or inter-individual variation. Usually when you take a pool of cells for any assay, you would average out the properties of individual cells. Using sequencing you may actually be able to ...


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Using the SeqRecord object approach with Bio.SeqIO, you would do something like this: my_record.annotations["data_file_division"] = "PLN" my_record.annotations["date"] = "24-DEC-2015" Note that only valid NCBI GenBank divisions, or their EMBL equivalent, will be accepted.


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Unfortunately, it does depend on sequencing techniques. For example, in Illumina sequencing, each sequence fragment is amplified (in order to get a stronger signal) and forms a cluster on the microarray. Each cluster is sequenced by cycles of: Adding fluorescent terminator nucleotides. These nucleotides are modified to contain an inhibiting/terminating ...


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This is the best review I have seen with a comprehensive discussion on gene duplication and amplification mechanisms (and it is also quite recent). It seems like there may be no definitive answers to your question in the literature--perhaps because of a paucity of viable experimental models that would allow one to test various hypotheses. Also @canadianer ...



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