Next-Gen Sequencing of RNA sample enriched with small RNAs (~15-200nt) such as miRNA, piRNA etc.
This technique is employed to detect and quantitate small RNAs such as miRNA, piRNA, endo-siRNA etc. The basic process involves:
- Enrichment of small RNAs using polyacylamide gel electrophoresis or RNA-binding columns
- Ligation of 3'and 5'adapters
- cDNA synthesis
- PCR amplification and library preparation