This technique is employed to detect and quantitate small RNAs such as miRNA, piRNA, endo-siRNA etc. The basic process involves:

  1. Enrichment of small RNAs using polyacylamide gel electrophoresis or RNA-binding columns
  2. Ligation of 3'and 5'adapters
  3. cDNA synthesis
  4. PCR amplification and library preparation
  5. Sequencing
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