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Necrotic cells could be trypan blue positive. WST-1 Assay could reflect cell number by measuring mitochondrial dehydrogenase, therefore, you could not know how many cells die in dishes. When you define apoptosis in your experiment as caspase dependent cell death, measuring caspase activity is the best. PS Trypan Blue Staining actually is a method to see ...


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The secretion pushes the cell organelles to the basal part. (although the fat cells have no polarity, their organelles have been pushed to one side of the cell: Comparing to adipose cells, the acinar cells secrete their specific proteins on one specific end of the cell, this is why in contrast with fat cells they have well defined polarity.



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