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Sample 1. 4096 / 1024 = 4x more B Sample 2. 16384 / 1024 = 16x more B The average amount more B = (16 + 4) / 2 = 10x more B That is not the right way to go about. You are calculating average expression of B in both samples. It does not mean there is 10x more B. There is just 4x more B in Sample-2 relative to Sample-1. Gene-A does not change ...



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