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You have a few misconceptions about stem cells, I will try to explain where they are. First of all, cells are not independent. They influence each other with signals and secreted messenger substances. If you look at a human embryo the state of totipotency (where all cells can differentiate into each cell type of the body) ends after 3-4 days when the ...


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When you look at the development of the embryo, at the beginning all cells are totipotent, meaning they can develop into any cell type of the body. This changes relatively fast by differentiation, which means that the totipotent cells develop into more specialized cell types, which then can not give rise to all cell types. So the different germ layers (meso, ...


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There is no specific dye for stem cells. You would have to do a immuno-histochemical staining for stem cell markers such as Sox2/Oct4 etc. Usually stem cells have a distinct morphology (round and clustered). You can use Leishman's (or Romanowsky-Giemsa) stain.


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It would not be possible to differentiate CSC from normal population non-invasively and select them out. You may do a single cell expression analysis to say if a CSC is present in a population or not but there is no magic bullet method for eliminating them. Also there are several oncogenes and some of them are also required for usual stem cell function. HAT ...


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Yes, stem cells can pass through blood vessels and capillaries (as @WYSIWYG points, these cells should be small enough to fit inside that capillary). The interesting thing is that they posses multiple mechanisms of transmigration. They are attracted by TNF-alpha activated endothelial cells [1] and can pass through by [1]: leukocyte-like diapedesis ...


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Signalling in the TGFβ pathway normally puts a brake on the progress of cells through the cell cycle - it counteracts the action of myc for example, partly by regulating the expression of myc. In Burkitt's lymphoma myc escapes this regulation because of a chromosomal translocation, tipping the regulatory balance over to uncontrolled cell growth. This ...


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Stem cells persist because they have the capacity to self-renew. At birth you will have a finite number of each stem cell type (e.g. hematopoietic stem cells that make blood, gut epithelium stem cells that make gut lining etc). The general picture is that as tissues require replenishment, a stem cell divides. One daughter cell starts to differentiate (and ...


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Sigma gives a solubility of 200 microMole in water containing 2% ethanol. They state: Forskolin is soluble in water (with 2% ethanol) up to 0.2 mM by first dissolving in ethanol at 5 mg/mL and doing subsequent dilutions with water. The datasheet can be found here.


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Strictly speaking ES cells are not man made, they come from blastocysts so already exist in nature. One thing you're forgetting is that even though stems cells that come from adults(experience aging) like iPS cells , they are reprogrammed to a naive or new state and thus are thought to "start all over" including getting longer telomeres(which shorten as you ...



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