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In general, there is no need for a spacing between stop codon and terminator. They can be right next to each other. When transcribed, transcription terminator forms a hairpin-like structure which interacts with the RNA polymerase to stop the transcription. Usually, the upstream sequence will not interact with the terminator and it will be transcribed ...


You can read more about Synthetic Biology at the iGEM Registry help pages. Also take a look at Cytostudio IDE and the Synthetic Bio-Programming language. A programming language and a compiler for Synthetic Biology

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