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Let's imagine that we understood DNA programming and our genome very well and >realized that there were some significant flaws (we die, we need sleep, etc.) And >let's imagine that we understand how to make our genome do what we want, but it's a >major refactor. We're going to have adjust 10-15% of our DNA. I think we both recognise that this is a very ...


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In a side-by-side comparison of 3 viral proteins (2 from paramyxoviridae, 1 from caliciviridae) each with both a "maximizing" and "matched" codon bias, we found the "maximized" version produced more protein the "matched" version in each instance. Protein levels were quantified via Western and flow, cell lines tested were 293T, HEp2 (HeLa contaminate), and ...


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Look into site-specific recombination. You can use a site-specific recombinase, specifically an integrase, that can insert a sequence of DNA at a certain attachment site. You can add an identical attachment site into the DNA sequence to insert, allowing for the reintegration of a new attachment site. Note however that you might get SSR onto the insertion ...



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