New answers tagged

1

I think the presence of a single terminator is related to the high processivity of T7 RNA polymerase. T7 RNA polymerase will transcribe a circular plasmid repeatedly before dissociating. The T7 terminator after ORF2 ensures that the transcript terminates efficiently. Based on your schematic, these are the potential mRNA transcripts from that construct: ...



Top 50 recent answers are included