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In general, there is no need for a spacing between stop codon and terminator. They can be right next to each other. When transcribed, transcription terminator forms a hairpin-like structure which interacts with the RNA polymerase to stop the transcription. Usually, the upstream sequence will not interact with the terminator and it will be transcribed ...


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You can read more about Synthetic Biology at the iGEM Registry help pages. Also take a look at Cytostudio IDE and the Synthetic Bio-Programming language. A programming language and a compiler for Synthetic Biology



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