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Take a look at this schematic of a mature mRNA. [source] The coding region (ie the part that is translated) is between the start and stop codons, but the 5' and 3' untranslated regions (UTRs) are also transcribed by RNA polymerase; these are part of the first and last exons, respectively. The transcription start site is labelled right in front of the 5' ...


4

You mix up translation and transcription. Transcription creates mRNA from DNA template. Transcription also includes splicing, that is excision of introns so that mature mRNA contains only exons. In your example it goes like that: DNA (chromosome): ---A----B--...--Dstop---E--- premature mRNA: A----B---...---Dstop--E---polyA mature mRNA: AB..DstopE-polyA ...


0

Nice question! First of all make sure that you have multiple transcription stop / terminator sequences at the end of your first gene . This is quite standard procedure. Also in your case the phenomenon called transcription interference (TI) comes handy. Here's a review on TI. In short: the term TI usually refers to the direct negative impact of one ...


3

Yes, transcription of a polyA sequence will be unreliable: for a repetition of 15 times the same nucleotide, the rate of transcription errors will be huge. For example in E. coli, this paper http://www.ncbi.nlm.nih.gov/pmc/articles/PMC331007/ gives some order of magnitude of transcription errors caused by repeats of nucleotides. According to their data, for ...


0

This article suggests RNA Pol III formed early in eukaryote evolution, and while there's a lot of conservation, the enzyme-specific subunits result from divergent evolution. Another article suggests the actual increase of subunits, however, is a result of the permanent recruitment of transcription factors to the core RNAP by studying homology of subunits to ...


2

The three RNA polymerases (RNAPs) are very similar to each other, yet not identical. As described in this article, there are subunits that are specific for each type of polymerases. In addition to providing unique substrates that polymerase-specific subunits bind to give each of the RNAPs their specific functionality, the two largest subunits also shape ...



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