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From the Methods section: Human TfR in plasmid cDNA was a gift from Tim McGraw (Weill-Cornell Medical College, New York, NY). Human TfR cDNA was subcloned in frame with EGFP in the Clontech pEGFP-N1 vector at the XhoI and BamHI restriction sites. This TfR-GFP fusion protein does not have the endogenous TfR promoter. So it is not likely to be ...



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