New answers tagged western-blot
Vinculin! I love our grad-students, I can't believe I didn't think of it last night. Also, awesome chart, even if it's from a company.
So we went ahead and did this. The efficiency was much lower, which you might expect, and dehydration did aid the process. We found that taking raw lysates and running them through the linked protocol, had about ~20% the efficiency of conducting it on purified protein. I assume the majority of the loss was due to reagents being expended on proteins that ...
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