Tag Info

It depends upon exactly what you have done. The standard way of using a yeast intergrating plasmid (YIp) is for it to integrate into the genome by recombination between a piece of yeast DNA that the YIp carries and the same DNA in the genome. This is often, but not necessarily, the selectable marker. So for example a YIp carrying the URA3 gene as its only ...

I think that you could try a similar approach to GSFS: use transduction in proteins (if you don't know star code, then you must use 3 strings for each gene) use a basic tool (a stand alone like UNIPROT tools) to identify the proteic domain type (chain alpha, ..) divide the genes by proteic domain type (pdt): which contains which pdt and the pdt order ...

Yeast integrating plasmids are known to be stable, even in absence of a selective medium but can revert like most homologous recombination plasmids. Quoting from the book Yeast Gene Analysis (pg476): YIps are generally more stable than YRp or YEp plasmids. As a result it is safe to grow most YIp-bearing strains in rich media, although it is good ...

According to this site, for yeast (I assume this means Saccharomyces cerevisiae) you should use 50 µg/ml to 200 µg/ml; for fungi (!) use 100 µg/ml to 300 µg/ml. The site also stresses the importance of the pH of the medium. I think that, unless a Hansenula expert comes along, you will have to try an initial experiment to measure the sensitivity of ...