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Jun
14
comment Designing genes with DNAWorks: Maximum nonzero score?
The gene is ~600 bp in size. I'm flexible on the oligo length and overlap length, but I was thinking something like 50-60 bp oligos and 15 bp overlaps. I'll assemble it by Gibson assembly directly into the vector backbone, but the part is designed so that I can also assemble it the standard way: first assembling the gene by PCR and then ligating it into the vector. Thanks!
Jun
13
awarded  Student
Jun
13
asked Designing genes with DNAWorks: Maximum nonzero score?