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8h
comment Is NADPH salt still usable after an hour at room temperature?
The question I posed is what is the difference between hydrated and unhydrated NADPH other than water of hydration. I am aware of the distinction that your chemist friend makes. You say NADPH is unstable in water. What is the breakdown product? Does it cause bleaching of the A340? The only reaction of NADH with water that I know of is the enzymic hydration of the 5,6-double bond.
9h
comment Doing a medical study on yourself?
A very famous experiment (Shemin and Rittenberg) to calculate the half-life of the red blood cell (120 days, or thereabouts) involved Shemin ingesting 66g of [15N]glycine. See here. (A note for molecular biologists: N-15 is NOT radioactive]
9h
comment Doing a medical study on yourself?
@ user137 Was it Linus Pauling? :-)
9h
revised Is NADPH salt still usable after an hour at room temperature?
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10h
revised Is NADPH salt still usable after an hour at room temperature?
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10h
comment Are trees the only source of large amounts of oxygen?
(+1) And, of course, the oxygen comes from the splitting of water.
10h
comment Are trees the only source of large amounts of oxygen?
One of the great discoveries of biology is that the oxygen evolved in photosynthesis comes from water not CO2. How do trees release 'the O from the CO2 into the atmosphere'??
10h
revised Is NADPH salt still usable after an hour at room temperature?
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10h
comment Is NADPH salt still usable after an hour at room temperature?
What exactly is the difference between 'hydrated' and 'dehydrated' NADPH, other than water or hydration, and how does this give rise to differences in stability? I see no water molecules in either of the Sigma diagrams you link to.
10h
comment Is NADPH salt still usable after an hour at room temperature?
In addition to oxidation, it is probably worth mentioning that the acid rearrangement product does not absorb at 340nm.
11h
revised Is NADPH salt still usable after an hour at room temperature?
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revised Is NADPH salt still usable after an hour at room temperature?
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22h
answered Is NADPH salt still usable after an hour at room temperature?
Jul
25
comment Why do Shiitake mushrooms grow on logs?
@MattDMo. One reasonable hypothesis is that shiitake mushrooms contain an enzymic system or enzymic systems capable of degrading lignin.
Jul
25
comment Why do Shiitake mushrooms grow on logs?
@MattDMo. Using your logic, you can explain anything. Why do eggs contain avidin? Because they do. Why do some bacteria contain streptavidin? Because they do. Why is the strepavidin-biotin bond one of the strongest non-covalent interactions known? Because it is.
Jul
16
comment Where is ATP used in Phosphorylation?
Oxidative phosphorylation is a mechanism that aerobic cells use to make ATP. As canadianer eludes to, ATP may also be 'made' by substrate-level phosphorylation. This is what happens in glycolysis, where the pyruvate kinase reaction is a good example of substrate-level phosphorylation 'making' ATP.
Jul
15
answered Is NADPH unstable in UV light?
Jul
14
awarded  Yearling
May
15
answered Any good website/book to understand protein folding and enzymes?