network profile
| bio | website | |
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| location | ||
| age | ||
| visits | member for | 9 months |
| seen | Aug 28 '12 at 18:49 | |
| stats | profile views | 4 |
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Aug 20 |
asked | What are the units of enzyme activity? |
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Aug 17 |
asked | What is the concentration of ATP in an average cell? |
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Aug 12 |
awarded | Nice Question |
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Aug 11 |
awarded | Scholar |
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Aug 11 |
awarded | Supporter |
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Aug 11 |
accepted | How would I explain different properties of the same protein in different species? |
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Aug 11 |
comment |
How would I explain different properties of the same protein in different species? Some very good ideas, thank you! Another interesting thing I forgot to mention, is that I found evidence of substrate inhibiton of C. albicans at about 1mM ATP concentration. Perhaps this would add some valuable knowledge to the evolutionary relationship between these orthologs? |
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Jul 30 |
comment |
How would I explain different properties of the same protein in different species? Do you know any databases where I could find the amino acid sequences of these protein orthologs? |
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Jul 30 |
revised |
How would I explain different properties of the same protein in different species? added 109 characters in body |
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Jul 30 |
comment |
How would I explain different properties of the same protein in different species? (i) I used an NADH-PK Linked Assay where I looked at the absorbance decrease when NADH is oxidized to NAD+. They were all pure proteins. (see: NADH-coupled microplate photometric assay for kinetic studies of ATP-hydrolyzing enzymes with low and high specific activities) (ii) All the Km values were different, ranging from 2mM-4mM and I used ATP concentrations ranging from 0mM to 10mM. |
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Jul 30 |
awarded | Student |
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Jul 30 |
awarded | Editor |
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Jul 30 |
comment |
How would I explain different properties of the same protein in different species? Yep, it was done in vitro. |
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Jul 30 |
revised |
How would I explain different properties of the same protein in different species? added 37 characters in body |
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Jul 30 |
asked | How would I explain different properties of the same protein in different species? |
