121 reputation
16
bio website
location
age 18
visits member for 2 years, 2 months
seen Mar 10 at 17:58

Sep
12
awarded  Notable Question
Nov
14
awarded  Popular Question
Aug
20
asked What are the units of enzyme activity?
Aug
17
asked What is the concentration of ATP in an average cell?
Aug
12
awarded  Nice Question
Aug
11
awarded  Scholar
Aug
11
awarded  Supporter
Aug
11
accepted How would I explain different properties of the same protein in different species?
Aug
11
comment How would I explain different properties of the same protein in different species?
Some very good ideas, thank you! Another interesting thing I forgot to mention, is that I found evidence of substrate inhibiton of C. albicans at about 1mM ATP concentration. Perhaps this would add some valuable knowledge to the evolutionary relationship between these orthologs?
Jul
30
comment How would I explain different properties of the same protein in different species?
Do you know any databases where I could find the amino acid sequences of these protein orthologs?
Jul
30
revised How would I explain different properties of the same protein in different species?
added 109 characters in body
Jul
30
comment How would I explain different properties of the same protein in different species?
(i) I used an NADH-PK Linked Assay where I looked at the absorbance decrease when NADH is oxidized to NAD+. They were all pure proteins. (see: NADH-coupled microplate photometric assay for kinetic studies of ATP-hydrolyzing enzymes with low and high specific activities) (ii) All the Km values were different, ranging from 2mM-4mM and I used ATP concentrations ranging from 0mM to 10mM.
Jul
30
awarded  Student
Jul
30
awarded  Editor
Jul
30
comment How would I explain different properties of the same protein in different species?
Yep, it was done in vitro.
Jul
30
revised How would I explain different properties of the same protein in different species?
added 37 characters in body
Jul
30
asked How would I explain different properties of the same protein in different species?