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location Canada
age 31
visits member for 2 years, 11 months
seen Oct 29 at 18:36

PhD Student


Aug
15
comment Optimization of annealing temperature
Posting the primer sequences and what genome they are designed against would be helpful. Furthermore, have you double checked that your primers are in the correct orientation?
Jul
26
comment Drosophila reference genome
As far as I know they just used a generic D. melanogaster lab strain.
Jul
26
comment Drosophila reference genome
Did you check the genome publication
Jul
21
comment Sequence of ribosomal RNA
@KonradRudolph: Oh perhaps these can be used as alternatives then Thermoscript or Affinity Script
Jul
19
answered Sequence of ribosomal RNA
Jul
11
revised Why does Penicillin only affect bacterial cell walls
added 35 characters in body
Jul
11
awarded  Cleanup
Jul
11
revised Why does Penicillin only affect bacterial cell walls
rolled back to a previous revision
Jul
11
awarded  Enlightened
Jul
11
awarded  Nice Answer
Jun
21
awarded  Enlightened
Jun
21
awarded  Nice Answer
Apr
29
awarded  Enlightened
Apr
29
awarded  Nice Answer
Apr
25
comment Difference between strand-specific and not strand-specific RNA-seq data
Yes, assuming that the libraries were prepared using a directional library.
Apr
25
comment Difference between strand-specific and not strand-specific RNA-seq data
No, after alignment the reads derived from the antisense strand should only align to the antisense strand of the genome (the same for reads from the sense strand). With a standard RNA-seq preparation reads could align to either strand, which prevents you from inferring the actual strand the read is derived from.
Apr
25
comment Difference between strand-specific and not strand-specific RNA-seq data
You mean aligning the reads?
Apr
25
answered Difference between strand-specific and not strand-specific RNA-seq data
Apr
25
answered Purpose of poly(A)+ RNA?
Apr
25
comment Majority of transcripts are from sense strand?
Could you elaborate on your question? Anti-sense transcripts are also transcribed in the 5'->3' direction. What type of data are you looking at?