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| bio | website | |
|---|---|---|
| location | Canada | |
| age | 29 | |
| visits | member for | 1 year, 6 months |
| seen | yesterday | |
| stats | profile views | 29 |
PhD Student
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May 16 |
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What material is recommended for autoradiography cassettes when working with 32P? Have you ever checked how much radiation passes through a cassette? |
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Feb 5 |
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What is the difference between SOLiD, 454, and Illumina next-gen sequencing? I think this question is a bit broad and perhaps you should read through the manuals / data sheets for the respective products. |
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Jan 10 |
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Can a tumor produce something not currently found in our own bodies? Isn't a tumour by nature something not inherently found in our body? |
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Dec 19 |
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Can paper/plastic currency serve as a medium for pathogens? It's going to vary wildly, for example Hepatitis B can survive 7 days outside of a host. Furthermore, some bacteria such as Bacillus antracis can produce endospores and survive for years. |
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Oct 9 |
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Sparking during electroporation of plasmid DNA into bacterial cells What media are the bacteria in during electroporation? |
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Sep 5 |
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Is “computational biology” different from “bioinformatics”? @KonradRudolph: Again the goal of my answer was not to give a concrete definition hence why I gave an additional note showing other people have varying opinions on what they are. The definitions are going to be very institute / lab specific depending on what their research topics and goals are. |
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Aug 15 |
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Optimization of annealing temperature Posting the primer sequences and what genome they are designed against would be helpful. Furthermore, have you double checked that your primers are in the correct orientation? |
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Jul 26 |
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Drosophila reference genome As far as I know they just used a generic D. melanogaster lab strain. |
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Jul 26 |
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Drosophila reference genome Did you check the genome publication |
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Jul 21 |
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Sequence of ribosomal RNA @KonradRudolph: Oh perhaps these can be used as alternatives then Thermoscript or Affinity Script |
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Jul 4 |
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What is RNAi and how is it useful? I really think this question could be answered by wikipedia, perhaps you could ask specific questions about what you don't understand? |
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Apr 25 |
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Difference between strand-specific and not strand-specific RNA-seq data Yes, assuming that the libraries were prepared using a directional library. |
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Apr 25 |
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Difference between strand-specific and not strand-specific RNA-seq data No, after alignment the reads derived from the antisense strand should only align to the antisense strand of the genome (the same for reads from the sense strand). With a standard RNA-seq preparation reads could align to either strand, which prevents you from inferring the actual strand the read is derived from. |
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Apr 25 |
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Difference between strand-specific and not strand-specific RNA-seq data You mean aligning the reads? |
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Apr 25 |
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Majority of transcripts are from sense strand? Could you elaborate on your question? Anti-sense transcripts are also transcribed in the 5'->3' direction. What type of data are you looking at? |
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Apr 4 |
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Why are some bodily fluids more of an infection risk than others? I feel like sputum / mucus should be on that list. It's the primary bodily fluid that influenza and other respiratory tract infections are spread with. |
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Mar 28 |
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Why does Penicillin only affect bacterial cell walls @bobthejoe: I updated my answer to better explain it. Thanks. |
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Mar 21 |
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Why does Penicillin only affect bacterial cell walls @RoryM: That is not totally correct, penicillin does affect some gram negative bacteria but it is not very effective. There are other ß-lactams that do affect gram-negative bacteria such as Carbenicillin. |
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Mar 21 |
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Do larger multicellular organisms have an increased risk of mutation and thus cancer? You also have to take into account that a lot of larger organisms have a large genome with a low percentage actually encoding exons. |
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Feb 27 |
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How to create a collection of anonymous sequences for teaching and testing? I think you need to elaborate more on what you are trying to accomplish. Furthermore, you may get more help on biostar |
