Absorption ratios 260/280 and 260/230 for RNA Indeed I remember than the sample with the ratio 260/230 had two peaks. In my case the values for the 260/230 ratio are never higher than the 260/280 which indicate I have contamination. What can be the consequences not to have the correct values for these ratio for a PCR? I will extract RNA soon. How can I prevent a contamination next time? What is the good practice?
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