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From the era before Sanger sequencing was invented.


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revised Do alternative start codons code for methionine after transcription?
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comment How do I interpret this graph regarding introduced genes and virus-infected cells?
For -/- and +/+ see this recent answer: biology.stackexchange.com/questions/20593/what-is-an-ihh-mouse/…
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revised How to stop or slow down the process of growth of hairs?
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answered Do alternative start codons code for methionine after transcription?
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revised Do alternative start codons code for methionine after transcription?
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comment Why isn't Bubonic Plague as virulent as it once was?
@har-wadim - very interesting - thanks!
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comment Do alternative start codons code for methionine after transcription?
Duplicate biology.stackexchange.com/questions/604/… also biology.stackexchange.com/questions/1629/… It seems to be the case these codons are still recognised by fMet-tRNA in the context of a translational start. See also WP en.wikipedia.org/wiki/Start_codon
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answered Why isn't Bubonic Plague as virulent as it once was?
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comment Regarding the difference between cDNA library and RNA sequencing (Biochem. technique)
PCR is carried out with a DNA polymerase, which can only deal with DNA as a template. The exception to this is of course reverse transcriptase, but that can't be used in a PCR because it isn't thermostable and it can't copy the DNA copies that it makes from an RNA template, so exponential amplification cannot take place.
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comment Regarding the difference between cDNA library and RNA sequencing (Biochem. technique)
I mean that a cDNA library exists as something tangible, not just in a computer.
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answered How to estimate the DNA density in human sperm head?
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answered Regarding the difference between cDNA library and RNA sequencing (Biochem. technique)
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comment How to estimate the DNA density in human sperm head?
@Bez I assume the aim is to get pg per um3 or something similar.
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comment How to estimate the DNA density in human sperm head?
I don't understand what the value 4-5 microns refers to. If it is supposed to be a volume it should have units of volume.
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revised What triggers the release of gonadotrophin-releasing hormone with the onset of puberty?
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revised How do Type I restriction enzymes work?
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answered How do Type I restriction enzymes work?
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revised Can humans survive without consuming life?
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revised What are the atmospheric requirements for large dinosaurs?
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revised Can humans survive without consuming life?
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