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From the era before Sanger sequencing was invented.


2d
revised To what extent do domestic animals understand language?
edited body; edited title
Jul
17
comment Why doesn't honey spoil quickly?
see biology.stackexchange.com/questions/19744/…
Jul
15
answered What is this discarded exoskeleton from?
Jul
15
revised What is the name of this flower with purple leaves and orange inner part in NYC?
added 407 characters in body
Jul
15
answered What is the name of this flower with purple leaves and orange inner part in NYC?
Jul
14
comment Turning publicly available genome data into proteins
I think that you need to be more specific than "I downloaded a complete human genome". Was this an entire genome sequence, or a set of FASTA sequences corresponding to predicted proteins?
Jul
11
comment How big can cold-blooded animals get?
Wikipedia en.wikipedia.org/wiki/Physiology_of_dinosaurs#Metabolism looks like a good place to start.
Jul
11
comment The move from RNA to DNA and the necessity of reverse transcriptase
You may be interested in the paper here: jbc.org/content/275/33/25523.full.pdf . The authors find that single mutations in an RNA-directed RNA polymerase can reduce selectivity for an NTP substrate to the point that there is no significant preference for NTPs over dNTPs. However this comes at the cost of very much reduced rate of polymerisation.
Jul
11
comment The move from RNA to DNA and the necessity of reverse transcriptase
This argument seems to be very circular. In an RNA world there presumably wouldn't be any DNA polymerase at all - what we should be looking for is an enzyme that can make DNA using an RNA template - which is a reverse transcriptase. The key step in the evolution of such an enzyme would be switching from polymerising NTPs to polymerising dNTPs.
Jul
11
comment The move from RNA to DNA and the necessity of reverse transcriptase
@Chris yes, apologies for the ambiguity, OP asserts that standard DNA polymerase can use RNA template, but I would like to see the evidence for this.
Jul
11
comment The move from RNA to DNA and the necessity of reverse transcriptase
Could you give a reference for standard DNA polymerases using an RNA template?
Jul
11
comment Significance of synthesis of D-glucose in plants..?
@Chris has explained the advantage of using one isomer - why do you think that it would be advantageous to synthesise and use both?
Jul
10
comment Will a vasectomy prevent genetic engineering of a child?
DNA from which cells?
Jul
9
answered Will a vasectomy prevent genetic engineering of a child?
Jul
9
answered How long does it take for a cell to synthesize an enzyme?
Jul
7
revised How does a stonefish get oxygen out of water?
deleted 1 character in body
Jul
7
answered How does a stonefish get oxygen out of water?
Jul
6
awarded  Enlightened
Jul
6
awarded  Nice Answer
Jul
5
revised A question about IPTG induction
edited body