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From the era before Sanger sequencing was invented.


Feb
21
revised Rate of cell division in humans
added 82 characters in body
Feb
21
answered Primer design and BLAST E value stringency
Feb
21
revised Primer design and BLAST E value stringency
added 1 characters in body
Feb
21
answered Rate of cell division in humans
Feb
21
comment Difference between Cary-Blair and Amies transport media for Staphylococcus and Streptococcus?
I put this up because it clearly states that Cary-Blair medium is used for enteric samples. Whether you should use it for that purpose is not explained. Presumably the Staphylococcus and Streptococcus you refer to are not samples of that type, so you would use Amies.
Feb
21
revised M-type potassium channels by muscarinic receptors
added 8 characters in body
Feb
21
answered Difference between Cary-Blair and Amies transport media for Staphylococcus and Streptococcus?
Feb
21
revised Difference between Cary-Blair and Amies transport media for Staphylococcus and Streptococcus?
deleted 3 characters in body; edited title
Feb
21
revised Primer design and BLAST E value stringency
clarification and syntax
Feb
21
comment Why do mammals in the wild retain the “trait” of menstruating with blood?
I've always wondered the same thing about crying babies - would attract predators.
Feb
21
comment Genome Assembly Using Reads
I would guess that it would be all 4mers. In this case that would mean that CGTTCT would also yield GTTC. But as I say - this is a guess.
Feb
20
comment Prevalent large (>=90kDa) maintenance protein/loading control
How about RNA polymerase subunits - some of these are large. (In E. coli I used to use beta-beta prime for the same reason. Not particularly abundant but very distinctive.)
Feb
20
comment Sequence analysis software suites
Starting from today.
Feb
19
comment Sequence analysis software suites
Thanks for pointing this out.
Feb
19
comment DNA sequence from the middle of a gene
I've edited the sequence to repair the 5' and 3' ends of the bottom strand, which are now as was shown in the original version of the question.
Feb
19
revised DNA sequence from the middle of a gene
deleted 2 characters in body
Feb
19
comment DNA sequence from the middle of a gene
The open reading frame is the strand of DNA with the same sequence as the mRNA (with T>U). RNA polymerase makes that RNA using the other DNA strand, which is the template strand. If the dsDNA sequence is written in the conventional way with promoter to the left then the top strand is the coding strand and the bottom strand (running 3'>5') is the template strand.
Feb
19
answered DNA sequence from the middle of a gene
Feb
18
comment Why prefer $MgSO_4$ over $MgCl_2$ in recipies
Although calcium sulphate is only sparingly soluble.
Feb
18
comment Will we ever be able to read the thoughts from brain activity?
I'm not convinced that I have learned to read my own thoughts yet.