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From the era before Sanger sequencing was invented.


Feb
21
revised Difference between Cary-Blair and Amies transport media for Staphylococcus and Streptococcus?
deleted 3 characters in body; edited title
Feb
21
revised Primer design and BLAST E value stringency
clarification and syntax
Feb
21
comment Why do mammals in the wild retain the “trait” of menstruating with blood?
I've always wondered the same thing about crying babies - would attract predators.
Feb
21
comment Genome Assembly Using Reads
I would guess that it would be all 4mers. In this case that would mean that CGTTCT would also yield GTTC. But as I say - this is a guess.
Feb
20
comment Prevalent large (>=90kDa) maintenance protein/loading control
How about RNA polymerase subunits - some of these are large. (In E. coli I used to use beta-beta prime for the same reason. Not particularly abundant but very distinctive.)
Feb
20
comment Sequence analysis software suites
Starting from today.
Feb
19
comment Sequence analysis software suites
Thanks for pointing this out.
Feb
19
comment DNA sequence from the middle of a gene
I've edited the sequence to repair the 5' and 3' ends of the bottom strand, which are now as was shown in the original version of the question.
Feb
19
revised DNA sequence from the middle of a gene
deleted 2 characters in body
Feb
19
comment DNA sequence from the middle of a gene
The open reading frame is the strand of DNA with the same sequence as the mRNA (with T>U). RNA polymerase makes that RNA using the other DNA strand, which is the template strand. If the dsDNA sequence is written in the conventional way with promoter to the left then the top strand is the coding strand and the bottom strand (running 3'>5') is the template strand.
Feb
19
answered DNA sequence from the middle of a gene
Feb
18
comment Why prefer $MgSO_4$ over $MgCl_2$ in recipies
Although calcium sulphate is only sparingly soluble.
Feb
18
comment Will we ever be able to read the thoughts from brain activity?
I'm not convinced that I have learned to read my own thoughts yet.
Feb
18
comment What modern insects are not (flying OR descended from flying ancestors)?
This all very interesting (really) but I don't understand it getting accepted since it doesn't appear to be an answer to the question from OP - modern insects with no flight in their ancestry.
Feb
18
comment How to convert nanogram to picomole?
I don't think it would be appropriate to take account of the biotin, since the RNA concentration (ng/ul) has presumably been estimated by a method that only measures RNA.
Feb
16
comment Effect of respiration on Arterial pressure
Keep scrolling until you get to Figure 4.
Feb
16
comment Converting unit/mg or unit/ml into ng/ml, or vice-versa
You need a value for the specific activity of the purified enzyme, in units/ mg (or similar). Also you need to check on the definition of a unit of activity for your enzyme since this is empirically defined.
Feb
16
comment Effect of respiration on Arterial pressure
I did a Google image search for respiration AND "arterial pressure" and found lots of graphs including one at 69.36.35.38/accp/pccsu/… which may be what you are looking for
Feb
16
revised Do “transcription factor binding site” and “operator” mean the same thing?
added 13 characters in body
Feb
15
revised How can I fetch nucleotide sequences based on ENA accession numbers and positions?
spelling