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seen Sep 29 at 18:51

From the era before Sanger sequencing was invented.


Sep
19
comment Transmembrane Protein Problem
Ionic bonds and covalent bonds are stronger than hydrogen bonds which are stronger than weak bonds like van der Waals. The term 'weak' isn't usually used in connection with ionic bonds in my experience, which is why I was puzzled.
Sep
19
revised Regarding inherited fertility in animals
added 15 characters in body
Sep
19
comment Transmembrane Protein Problem
What is a weak ionic bond?
Sep
18
revised Why can't the immune system start at the mating time?
deleted 2 characters in body
Sep
17
revised Correlating beta-lactamase expression to absorbance
added 16 characters in body
Sep
17
revised Correlating beta-lactamase expression to absorbance
added 18 characters in body; edited title
Sep
17
comment Fermentation to acetic acid
A Google search for "homemade apple vinegar" yields lots of information. Perhaps you will get an answer from an expert here, perhaps not.
Sep
17
comment Why doesn't the cell membrane just… break apart?
Also the bilayer maximises van der Waals interactions between the closely-packed hydrocarbon chains, again contributing to the stability of the bilayer.
Sep
17
comment transfection of expression library made from cDNA into cells expressing the desired MHC haplotypes
I've looked at the paper, but it is simply a two page review - it doesn't describe any result related to the methodology that you describe as far as I can see (it only cites 14 references).
Sep
17
answered PDB file SEQRES have different datas?
Sep
17
comment What is the most critical step of plasmid purification?
@Chris I agree, I was trying to stress to the OP that the whole process should be carried out carefully, which is what you said. Remembering to add antibiotic to the cultures is also critical!
Sep
17
comment What is Colloidal biology and does it have any scientific background?
The WP page on colloids en.wikipedia.org/wiki/Colloid#In_biology has a useful jumping off point (see subheading "In biology"). There is a discussion in the book "A History of Molecular Biology" my Michel Morange.
Sep
17
revised How do I prepare a solution? (Calculation)
spaces between numbers and their units
Sep
17
comment What is the most critical step of plasmid purification?
So, in summary, all of the steps are critical.
Sep
17
comment Why doesn't the cell membrane just… break apart?
Perhaps worth adding that the formation of the bilayer increases the entropy of the water, but the entropy associated with the phospholipids will decrease. The net change in entropy is favourable.
Sep
17
answered How can I text-mine full-text articles from PubMed?
Sep
17
comment What is hTAP?? Please help
You definitely need to add some context - what sentence(s) did you see it in, and where? A Web Of Science search for hTAP in the TITLE field points to "Human thyrotropin-releasing hormone-associated peptide" but that doesn't appear to be relevant. It isn't unusual for acronyms like this to have several meanings.
Sep
17
revised How can I download a gene sequence from GenBank (NCBI)?
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Sep
16
revised Affinity isolated peptide
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Sep
16
comment Is it possible to calculate the energy required to speak a word?
It may be more useful to consider the total amount of sound required to convey information. It has always seemed to me that Italian is particularly profligate. Example chosen at random: bread maker (English, 3 syllables); macchina per il pane (Italian, 7 syllables). But, of course, this isn't really a biology question.