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From the era before Sanger sequencing was invented.


Aug
23
comment How do I interpret this graph regarding introduced genes and virus-infected cells?
For -/- and +/+ see this recent answer: biology.stackexchange.com/questions/20593/what-is-an-ihh-mouse/…
Aug
23
revised How to stop or slow down the process of growth of hairs?
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Aug
23
answered Do alternative start codons code for methionine after transcription?
Aug
23
revised Do alternative start codons code for methionine after transcription?
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Aug
23
comment Why isn't Bubonic Plague as virulent as it once was?
@har-wadim - very interesting - thanks!
Aug
23
comment Do alternative start codons code for methionine after transcription?
Duplicate biology.stackexchange.com/questions/604/… also biology.stackexchange.com/questions/1629/… It seems to be the case these codons are still recognised by fMet-tRNA in the context of a translational start. See also WP en.wikipedia.org/wiki/Start_codon
Aug
22
answered Why isn't Bubonic Plague as virulent as it once was?
Aug
22
comment Regarding the difference between cDNA library and RNA sequencing (Biochem. technique)
PCR is carried out with a DNA polymerase, which can only deal with DNA as a template. The exception to this is of course reverse transcriptase, but that can't be used in a PCR because it isn't thermostable and it can't copy the DNA copies that it makes from an RNA template, so exponential amplification cannot take place.
Aug
22
comment Regarding the difference between cDNA library and RNA sequencing (Biochem. technique)
I mean that a cDNA library exists as something tangible, not just in a computer.
Aug
22
answered How to estimate the DNA density in human sperm head?
Aug
22
answered Regarding the difference between cDNA library and RNA sequencing (Biochem. technique)
Aug
22
comment How to estimate the DNA density in human sperm head?
@Bez I assume the aim is to get pg per um3 or something similar.
Aug
22
comment How to estimate the DNA density in human sperm head?
I don't understand what the value 4-5 microns refers to. If it is supposed to be a volume it should have units of volume.
Aug
22
revised What triggers the release of gonadotrophin-releasing hormone with the onset of puberty?
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Aug
22
revised How do Type I restriction enzymes work?
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Aug
22
answered How do Type I restriction enzymes work?
Aug
21
revised Can humans survive without consuming life?
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Aug
21
revised What are the atmospheric requirements for large dinosaurs?
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Aug
21
revised Can humans survive without consuming life?
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Aug
21
revised What is a protective epitope?
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