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| location | United Kingdom | |
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| visits | member for | 8 months |
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From the era before Sanger sequencing was invented.
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May 14 |
comment |
Why is Sanger sequencing inferior for detecting SNPs in cancer cells? @WYSIWYG I'm not sure what either of these comments is adding. There is no question that Sanger sequencing can be used to derive genome sequences, since as you say it has been so used. My interpretation of this question was that it relates to a scenario in which a mixed sample is to be sequenced, which creates problems for using Sanger sequencing, as I have tried to explain. |
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May 14 |
answered | Why is Sanger sequencing inferior for detecting SNPs in cancer cells? |
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May 13 |
comment |
Breeding laboratory mice with cancer; how does this work? Just a very minor correction: although geneticin and G418 are the same antibiotic, neomycin is a different molecule (although they are both aminoglycosides). Resistance to both is conferred by an aminoglycoside 3' phosphotransferase, and the gene encoding this enzyme is often designated neo |
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May 13 |
revised |
What's the difference between shotgun sequencing and clone based sequencing? edited title |
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May 13 |
answered | By what mechanism does elevated homocysteine level accelerate thrombin formation? |
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May 12 |
revised |
Will lipid molecules 'flip-flop' over a membrane without the use of an enzyme? edited body |
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May 12 |
revised |
DNA replication Okazaki fragments edited title |
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May 12 |
comment |
DNA replication Okazaki fragments If I understand the question, this would require that the entire leading strand was copied first, and then the entire lagging strand would be copied using a primer put in at the terminus of leading strand replication. Apart from any considerations of keeping all of that single-stranded DNA safe from harm, this would mean that replication of an entire dsDNA molecule would take twice as long. |
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May 12 |
revised |
Will lipid molecules 'flip-flop' over a membrane without the use of an enzyme? edited body |
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May 12 |
answered | Will lipid molecules 'flip-flop' over a membrane without the use of an enzyme? |
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May 11 |
revised |
Why is elevated homocysteine considered a risk factor for angina? edited title |
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May 11 |
revised |
Why is elevated homocysteine considered a risk factor for angina? syntax |
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May 11 |
revised |
By what mechanism does an obstructed bile duct cause excess fat in the stool? edited body; edited title |
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May 11 |
revised |
Can I purify polyhydroxyalkanoates by heating the cells extensively? defined the abbreviation |
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May 10 |
awarded | Nice Answer |
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May 9 |
revised |
Does biofuel production by microalgae need an extra source of CO2? minor improvements to phrasing and spelling |
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May 8 |
revised |
Why do mammalian red blood cells lack a nucleus? edited title |
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May 8 |
revised |
Why do mammalian red blood cells lack a nucleus? deleted 1 characters in body |
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May 7 |
comment |
Are there more descriptive ways of naming genes and gene interactions? This is of only peripheral relevance, but I think that you have got the history of yeast genetic nomenclature backwards. For decades yeast genes were defined functionally and were named accordingly. So, for example, the CDC genes (uppercase = dominant form, usually wild-type, lowercase = recessive form, usually mutant) were described by Lee Hartwell in 1970, about 25 years before the yeast genome was sequenced. At this point ALL of the ORFs/genes were given a systematic name (e.g. CDC1 is YDR182W). The aim is, however, still for all genes to be given a name relating to function as well. |
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May 7 |
comment |
How are zooids identified? There are facts and ruminations at siphonophores.org/SiphOrganization.php From the link it would seem that all zooids in a single colony (animal) are genetically identical, having developed by division from a single zooid. |
