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From the era before Sanger sequencing was invented.


Nov
12
comment explanation of conjugation experiment in bacteria
Great answer - was working on an answer myself, but no need for it now. It's worth adding a historical note that this paper, and especially an earlier (1959) paper from Pardee, Jacob & Monod, formed the basis of the idea of mRNA - an unstable intermediate between gene (DNA) and protein. And this in turn led to the experiments of Nirenberg that are touched upon in this question
Nov
11
comment Homework : Sodium Potassium pump
I don't know the answer, but my feeling is that it will be (b). If the cell isn't a neuron then there will be no processes directly collapsing the resting potential. There will however be lots of symporters and antiporters carrying out secondary active transport by tapping into the gradients of sodium and potassium ions.
Nov
11
answered Proportion of amino acids from a random polymer of U and C
Nov
11
revised Why is the 4.4 kb band barely visible in this HindIII digest of lambda DNA?
deleted 22 characters in body
Nov
11
answered Why is the 4.4 kb band barely visible in this HindIII digest of lambda DNA?
Nov
11
revised Why is the 4.4 kb band barely visible in this HindIII digest of lambda DNA?
general tidy up
Nov
11
revised Why is the 4.4 kb band barely visible in this HindIII digest of lambda DNA?
edited body; edited title
Nov
10
revised What is one Botulinum toxin medical unit?
syntax improved
Nov
10
revised Why are interspecies hybrids so often larger than either parent species
spelling
Nov
9
revised What is one Botulinum toxin medical unit?
added 118 characters in body
Nov
9
answered What is one Botulinum toxin medical unit?
Nov
8
revised mRNA with Pseudouridine and 5-methylcytidine?
spellings and pedantry
Nov
8
revised Difference between CDS and cDNA
added 23 characters in body
Nov
8
comment Difference between CDS and cDNA
Yes, @terdon is correct - I'm so protein-oriented I overlooked this. So the cDNA sequence will encompass the CDS. Will add a correction to the answer.
Nov
8
comment Measuring algae growth or death
I agree with your answer, but it got me thinking. You could use a spectrophotometer if you took identical samples of algae, treated with different concentrations of agent, then inoculated an aliquot of each into a fresh culture (with a fairly large dilution). By reading the OD of these secondary cultures at some later time you could relate the final OD to how many living cells were inoculated. This could easily be standardised by setting up a calibration with different amounts inoculated from an untreated control.
Nov
8
answered Difference between CDS and cDNA
Nov
7
revised Density of neurons in the cortical column
tidy and spelling
Nov
7
comment Other than Acetylcarnitine what other orally-bioavailable Acetyl donors exist to assist in the conversion of CoA to acetyl-CoA?
Is there any evidence that acetylcarnitine acts as an "acetyl donor"? I understood that its use as a supplement was to supply carnitine. Could you give a reference for the statements in the second paragraph of the question?
Nov
7
revised Are wild cats the same species as house cats?
tidying
Nov
7
revised Are wild cats the same species as house cats?
spellings and pedantry