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From the era before Sanger sequencing was invented.


Jan
24
revised DNA ligase mechanism
added 19 characters in body
Jan
24
revised Reference sequence for defining single nucleotide polymorphisms
tidy and spelling
Jan
24
answered What is “transcribed strand”
Jan
22
revised How suddenly does lactose-intolerance onset?
added 542 characters in body
Jan
21
comment Identifying mutations in a yeast strain
Most deletions cannot revert because they represent a large loss of information. A deletion of a single base would cause a frameshift, and that could revert as a result of a compensating insertion (or two more single deletions) that restored a functional reading frame.
Jan
20
answered How suddenly does lactose-intolerance onset?
Jan
18
comment Process of Transcription
You've tagged this DNA, but really it is about RNA (although the same principles apply).
Jan
18
answered Process of Transcription
Jan
11
comment Topology of protein
This review (bio.ijs.si/~krizaj/group/Predavanja%202011/…) indicates that thioacylation of the type depicted in the question is indeed a cytoplasmic process.
Jan
11
comment Source of energy of adipocytes
In the fasting state blood glucose levels will be maintained by the liver via gluconeogenesis, so yes. During fasting the main function of the adipocytes is fat mobilisation and release of fatty acids (and glycerol, used by the liver as a substrate for gluconeogenesis).
Jan
10
comment Identifying mutations in a yeast strain
Just to add a useful term to this answer: the rare colonies on the -Trp plates and the -His at 37C are called revertants. Mutation classes (a), (b), (c) and (d) as listed in the question are all capable of reverting. A deletion cannot revert.
Jan
10
revised Identifying mutations in a yeast strain
deleted 7 characters in body
Jan
10
answered Source of energy of adipocytes
Jan
7
revised Gene transfer between Gram positive and Gram negative bacteria?
improved clarity especially the title
Jan
7
comment Lactate and Diabetes
Can you provide any references in support of your assertion that diabetes is associated with elevated levels of lactate?
Jan
6
answered On which strand does the promoter sit?
Jan
6
comment On which strand does the promoter sit?
@Chris I don't think it is correct to say that the coding strand is transcribed - the coding strand of the DNA has the same sequence as the RNA produced by transcription (except T>U). The other strand of the DNA is the template for the synthesis of the RNA.
Jan
6
comment Why are haploid cancer cells not killed by immune system?
I don't have an answer but - "Near-haploid karyotype in a squamous cell lung carcinoma" - onlinelibrary.wiley.com/doi/10.1002/gcc.2870070405/abstract
Jan
6
comment Is the complement system a part of innate or adaptive immunity
Just to underline that last point from @Chris - the signature property of the complement system is the use of proteins with reactive thioester groups to covalently tag cells, and this is very ancient. The plugging together of antibodies and the complement system is certainly a relatively recent innovation. The OP raises what is really a semantic point. There are also interactions between cellular immunity and humoral immunity, but it is still useful to think of these as separate processes with an interface.
Jan
6
revised Is the complement system a part of innate or adaptive immunity
grammar pedantry