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visits member for 1 year, 11 months
seen 18 hours ago

From the era before Sanger sequencing was invented.


Oct
23
comment How do trees lift water higher than 10 meters?
My last contribution - try this.. There is a whole literature on this topic out there, and I suggest that you explore it.
Oct
23
revised Criteria for the numbering of human chromosomes
spelling, clarity
Oct
23
comment How do trees lift water higher than 10 meters?
Maybe this discussion provides a better explanation of current thinking.
Oct
23
comment Does the Petri dish lid orientation on workbench affect aseptic technique?
@terdon I admit, I didn't watch it till the end - I just wanted a quick link to illustrate the idea.
Oct
23
comment Does the Petri dish lid orientation on workbench affect aseptic technique?
Depends what exactly you are doing - fungal contamination can also be a problem, especially when working with yeast.
Oct
23
comment Does the Petri dish lid orientation on workbench affect aseptic technique?
@terdon You don't have to pick out the beads. Once you have "spread" you invert the plate as usual and they fall into the lid. After incubation,as you check the plates you can tip the beads out for reuse. I put beads onto plates before adding the liquid - the lid is opened slightly for a second or two. (I autoclave beads in glass tubes and tip a few on to each plate). The main advantage of the method (apart from no alcohol, no disposable plastic) is in plating a whole stack of plates: add beads, pipette samples and then swirl the stack all at once. Really, I recommend that you try it out.
Oct
22
comment Euthyrodism and goitre
I don't know the answer, but here is a fairly recent and highly-cited review that may be a good place to start: Krohn et al. (2005) Molecular pathogenesis of euthyroid and toxic multinodular goiter. Endocrine Reviews 26: 504-524
Oct
22
comment pH inside the Giardia intestinalis cells
Google to the rescue: search = giardia intracellular pH, top hit is jbc.org/content/276/31/29157.full a J Biol Chem paper which says in the Abstract that the intracellular pH is 6.7 - 7.1 under the conditions studied.
Oct
22
comment Function of carapace
I don't know anything about this topic, but surely the key to this is that the question is actually asking about processes extending from the modified carapace, so this is function over and above exoskeleton function. I can imagine that such processes could help with dispersal in currents.
Oct
22
comment Does the Petri dish lid orientation on workbench affect aseptic technique?
If you are spreading then there isn't any need to put the lid down. One hand simultaneously opens the lid and holds the dish, the other does the spreading. That's what I used to do. However the best solution is to use glass beads - see youtube.com/watch?v=TACbj-ixW-U although ironically I don't think much of his lid technique!
Oct
21
comment Whole genome amino acid composition tool?
Just to amplify the comment from @terdon - do you mean the amino acid composition of the proteome (i.e. taking into account protein abundance) or do you mean that simply calculated from the translation of all open reading frames?
Oct
21
revised Lyonization and X-linked disorders?
added 2593 characters in body
Oct
21
comment How to measure bacterial content
Thanks to leonardo for answering this before I got a chance to!
Oct
20
comment Lyonization and X-linked disorders?
I don't know the answer, but I think that you are getting your diseases mixed up - it's sickle cell anaemia that's a haemoglobin disorder. I seem to recall however that the most common genetic disease in humans, glucose 6-phosphate dehydrogenase deficiency (X-linked) is an example of a blood disorder in which heterozygous females have two populations of red cells.
Oct
20
revised Sources for common laboratory Saccharomyces strains?
typo
Oct
20
answered How to measure bacterial content
Oct
20
comment Good book for aquarium owning?
Sounds very ambitious to me.
Oct
20
answered Question about production of CFTR protein
Oct
20
comment Ideal blood cell
Is it intended to be biconcave, like a red cell? If so it would have difficulty carrying out the phagocytic functions of some of the other cell types I have to say I don't understand the purpose of this assignment.
Oct
20
answered Lyonization and X-linked disorders?