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From the era before Sanger sequencing was invented.


Jun
13
comment level of organisation of proteins
Apologies Shigeta, I just didn't see it, possibly because I was still puzzled by the term 'intra-domain". I know what that means, but it doesn't map on to quaternary in my opinion.
Jun
12
comment level of organisation of proteins
Surely immunoglobulin molecules are excellent examples of the involvement of disulphides in the quaternary strucure of proteins?
Jun
12
comment What percentage of chimeric takes up in a cDNA library?
You say that you have looked at three frames. Which end of the cDNA are you sequencing from - are you sure that you are translating the correct strand?
Jun
11
comment How do penicillin resistant bacteria grow slower in the presence of penicillin?
Resistance to penicillin in B cereus is due to chromosomal genes, but not all B cereus strains are penicillin resistant. Do you know for certain that you used a resistant strain?
Jun
11
comment How does heat shock transformation work?
I think it is still true that no-one knows "exactly" what happens. There is a piece of received wisdom that by growing cells at 37C then shocking them at 42C, the membrane becomes more fluid (true) and this helps DNA to cross this barrier. If anyone writes an answer with evidence for this I for one will be very interested.
Jun
11
awarded  Custodian
Jun
11
revised alpha tubulin molecular weight problem
edited title
Jun
10
answered Can TGF beta family induce all somatic stem cells?
Jun
10
answered What are the naming conventions for mutations of proteins?
Jun
10
revised Silene virginica Endangered in FL
edited body
Jun
10
answered Breaking a gene during recombination
Jun
10
answered How many recombination events are there per generation in humans?
Jun
6
revised On which part of the DNA will the new DNA nucleotide connect?
added 1 character in body
Jun
6
comment Why does the oxygen produced in the photosynthesis come from water and not CO$_2$?
@The Last Word - great link.
Jun
6
revised Why does the oxygen produced in the photosynthesis come from water and not CO$_2$?
edited body
Jun
6
revised Anaerobic respiration choice in E. coli
deleted 5 characters in body
Jun
6
revised Why are some chemical dangerous to aquatic environment only?
added 16 characters in body
Jun
4
revised Epistasis Involving Multiple Loci
edited title
Jun
4
revised What does “thermodynamic equilibrium” mean for an enzyme-substrate complex?
edited body
Jun
3
comment Diffusion of Hypotonic solution into solution with glucose and starch
But, by definition, if the glucose concentration is higher on the outside then the rate of movement inwards will be greater than the rate of movement outwards. In my view the presence of the non-diffusible solute inside the bag will cause a net movement of water into the bag until this is exactly counteracted by the pressure created. The glucose is simply a passive observer and will equilibrate (equal concentration inside and outside). As I say, this is my view, and I am happy for someone to explain why this is incorrect.