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seen Sep 29 at 18:51

From the era before Sanger sequencing was invented.


Sep
10
comment Is there sugar secreted into blood even after complete digestions in healthy humans? What about dogs?
I don't understand the question. Both humans and dogs use blood glucose to supply tissues. Glucose is always entering the blood stream, either directly from the gut or from the tissues which manufacture glucose from precursors (i.e. the liver and the kidney). This is a continuous process since it is necessary to offset the glucose being used by various tissues, especially the brain. The problem in diabetes is that this balance is undone, but the defect is on the consumption side.
Sep
9
revised Can a single axon propagate multiple simultaneous action potentials?
edited title
Sep
8
comment Enzyme Assay - pectinase
pretreat the enzyme at high temperature before carrying out the assay at the standard temperature
Sep
8
revised Why do I see so many handicapped dorbeetles?
edited title
Sep
8
comment What is the difference between second and third generation sequencing
@har-wadim thanks, I hadn't thought of it like that. What increase in throughput constitutes a generation?
Sep
7
comment What good is the MinION?
Also omicsomics.blogspot.co.uk/2014/09/…
Sep
7
comment What good is the MinION?
This blog post may be useful biomickwatson.wordpress.com/2014/09/07/…
Sep
7
revised Why is it that bacteria and fungi cannot develop resistance against essential oils?
deleted 14 characters in body; edited title
Sep
7
answered Why is it that bacteria and fungi cannot develop resistance against essential oils?
Sep
7
revised What would cause crows to be attracted to a human body?
Removed quotation marks which seemed to serve no purpose in the context of the actual question.
Sep
7
comment What is the difference between second and third generation sequencing
Ultimately we, as scientists, shouldn't get hung up on the exact use of this terminology since it is clearly now just part of marketing the latest advance in sequencing technology.
Sep
7
comment What is the difference between second and third generation sequencing
In my opinion referring to Sanger sequencing as 'first generation' is historically incorrect. Maxam-Gilbert sequencing preceded it and produced lots of useful results - see Addendum in my answer here: biology.stackexchange.com/questions/17598/…
Sep
5
answered Protein Isolation from mammalian cells
Sep
5
revised Is there a difference between any of these terms for proteins: “crystal structure,” “NMR structure,” “solution structure,” and “complex structure”?
added 6 characters in body
Sep
4
revised What is most simple eukaryotic genome?
deleted 3 characters in body
Sep
4
answered How is it that ionic diffusion is independent of other ions?
Sep
4
answered Is there a difference between any of these terms for proteins: “crystal structure,” “NMR structure,” “solution structure,” and “complex structure”?
Sep
4
comment What is most simple eukaryotic genome?
...but M. genitalium is not a eukaryote!
Sep
3
comment What is this fuzzy, black fungus that grew on my plates in the 4⁰C room?
Yes I saw that. However fungal colony morphology is very dependent upon the composition of the agar. But if I was more confident I would have answered, not comented.
Sep
3
revised Has it been confirmed yet that the DNA found in dinosaurs was really DNA?
edited title