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seen Sep 29 at 18:51

From the era before Sanger sequencing was invented.


Sep
25
comment What do you call a virus when it's cured?
It is indeed "a certain type of organism". I think that you might find the Wikipedia page en.wikipedia.org/wiki/Virus useful. It certainly answers your question.
Sep
22
comment Where do the electrons and protons formed from biological reactions go?
@Chris thanks, I forgot about glutathione. Of course the diagram could actual be the oxidation of glutathione!
Sep
22
comment What governs the size of animal bodies?
For the record - both my comment and that from Mike Taylor make no sense now that the question has been edited. It originally mentioned wanting to ride a dinosaur.
Sep
22
comment What governs the size of animal bodies?
The blue whale en.wikipedia.org/wiki/Blue_whale is the largest animal that has ever existed on Earth, and it is available to ride right now.
Sep
20
comment How can I determine the purity of cells isolated from rat brains if I cannot use FACS, Immunohistochemistry or SEM anaysis?
I guess that a Western would count as an example of an immunological technique (excluded in the question).
Sep
20
comment Why can't a Corn have 13 rows of seeds?
There should be a way to give a double up vote.
Sep
20
comment Determining and Differentiating specific ATPases
Yes, but as I said in my answer, you can artificially get F-ATPase to do that, although probably not in the context of cellular compartments.
Sep
19
comment Transmembrane Protein Problem
Ionic bonds and covalent bonds are stronger than hydrogen bonds which are stronger than weak bonds like van der Waals. The term 'weak' isn't usually used in connection with ionic bonds in my experience, which is why I was puzzled.
Sep
19
comment Transmembrane Protein Problem
What is a weak ionic bond?
Sep
17
comment fermentation to acetic acid
A Google search for "homemade apple vinegar" yields lots of information. Perhaps you will get an answer from an expert here, perhaps not.
Sep
17
comment Why doesn't the cell membrane just… break apart?
Also the bilayer maximises van der Waals interactions between the closely-packed hydrocarbon chains, again contributing to the stability of the bilayer.
Sep
17
comment transfection of expression library made from cDNA into cells expressing the desired MHC haplotypes
I've looked at the paper, but it is simply a two page review - it doesn't describe any result related to the methodology that you describe as far as I can see (it only cites 14 references).
Sep
17
comment What is the most critical step of plasmid purification?
@Chris I agree, I was trying to stress to the OP that the whole process should be carried out carefully, which is what you said. Remembering to add antibiotic to the cultures is also critical!
Sep
17
comment What is Colloidal biology and does it have any scientific background?
The WP page on colloids en.wikipedia.org/wiki/Colloid#In_biology has a useful jumping off point (see subheading "In biology"). There is a discussion in the book "A History of Molecular Biology" my Michel Morange.
Sep
17
comment What is the most critical step of plasmid purification?
So, in summary, all of the steps are critical.
Sep
17
comment Why doesn't the cell membrane just… break apart?
Perhaps worth adding that the formation of the bilayer increases the entropy of the water, but the entropy associated with the phospholipids will decrease. The net change in entropy is favourable.
Sep
17
comment What is hTAP?? Please help
You definitely need to add some context - what sentence(s) did you see it in, and where? A Web Of Science search for hTAP in the TITLE field points to "Human thyrotropin-releasing hormone-associated peptide" but that doesn't appear to be relevant. It isn't unusual for acronyms like this to have several meanings.
Sep
16
comment Is it possible to calculate the energy required to speak a word?
It may be more useful to consider the total amount of sound required to convey information. It has always seemed to me that Italian is particularly profligate. Example chosen at random: bread maker (English, 3 syllables); macchina per il pane (Italian, 7 syllables). But, of course, this isn't really a biology question.
Sep
16
comment Knowledge about Organelles
E. coli, as a prokaryote, doesn't have mitochondria. It maybe better to think in terms of Saccharomyces cerevisiae (yeast).
Sep
15
comment Manufacturing of Bio Molecules
I don't have an answer to my reading of the question.