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|---|---|---|
| location | United Kingdom | |
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| visits | member for | 9 months |
| seen | 12 hours ago | |
| stats | profile views | 41 |
From the era before Sanger sequencing was invented.
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1d |
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In vitro enzyme production There are kits: Google "in vitro expression" and you will find that all of the major companies do one. I have no experience with any of them. |
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May 21 |
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Reverse complement of reconstruction model for assembling reads Yes, if you have a read (which will be by convention 5'>3') then you can search for it directly on the top strand of the target (also 5'>3'). If you need to look for it on the other strand of the target you can't simply search on the complementary because this is 3'>5', so you derive the reverse complement. It's just the equivalent of turning the dsDNA sequence (on the page) through 180 degrees. |
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May 21 |
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Reverse complement of reconstruction model for assembling reads Isn't this just a case of keeping everything straight with respect to 5'>3' direction? If a read matched "TGACAGG" this would not correspond to either strand of your example, because writing it like that implies that it is 5'-TGACAGG-3' unless it is made explicitly clear that it is the bottom strand of a duplex. |
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May 20 |
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Immunity during pregnancy In fact antibodies do cross the placental barrier: there is active transplacental transport of maternal IgG into the foetal circulation. |
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May 20 |
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Transplantation of stem cells Although most of these stem cells are in the bone marrow, there are also some in the blood and these are collected in order to perform an autologous transplant. |
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May 19 |
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Writing methods section on PCR amplication in a paper @Des-microbiology this site looks like a reasonable place to start. |
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May 18 |
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Wheat anthracnose outbreaks caused by Colletotrichum cereale in the USA The review you mention in the quotation (...which is from?...) is available here and seems to be a good source of at least some relevant references. |
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May 18 |
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Wheat anthracnose outbreaks caused by Colletotrichum cereale in the USA It's not clear which case you mean - 1940s or golf courses? |
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May 17 |
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Writing methods section on PCR amplication in a paper Is Tag meant to be Taq? |
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May 17 |
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Writing methods section on PCR amplication in a paper @terdon or even better, use the correct abbreviation for seconds which is s, not sec(s). And use min for minutes (never mins) |
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May 17 |
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Are there plants that excrete poisons into their environments? this is called allelopathy |
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May 17 |
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Primer design for Gibson assembly @WYSIWYG the TyrRs gene is just 1920 bp of the plasmid in the linked file. |
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May 16 |
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What is the purpose of Y-shaped adapters in Illumina sequencing? This looks like a nice explanation. |
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May 15 |
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In which blood vessels are these nutrients first found? According to Wikipedia the answer is the subclavian veins: "The thoracic duct drains into the left subclavian vein, near its junction with the left internal jugular vein. It carries lymph (water and solutes) from the lymphatic system, as well as chylomicrons or chyle, formed in the intestines from dietary fat and lipids. The right lymphatic duct drains its lymph into the junction of the right internal jugular vein, and the right subclavian vein." |
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May 14 |
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Why is Sanger sequencing inferior for detecting SNPs in cancer cells? @WYSIWYG I'm not sure what either of these comments is adding. There is no question that Sanger sequencing can be used to derive genome sequences, since as you say it has been so used. My interpretation of this question was that it relates to a scenario in which a mixed sample is to be sequenced, which creates problems for using Sanger sequencing, as I have tried to explain. |
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May 13 |
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Breeding laboratory mice with cancer; how does this work? Just a very minor correction: although geneticin and G418 are the same antibiotic, neomycin is a different molecule (although they are both aminoglycosides). Resistance to both is conferred by an aminoglycoside 3' phosphotransferase, and the gene encoding this enzyme is often designated neo |
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May 12 |
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DNA replication Okazaki fragments If I understand the question, this would require that the entire leading strand was copied first, and then the entire lagging strand would be copied using a primer put in at the terminus of leading strand replication. Apart from any considerations of keeping all of that single-stranded DNA safe from harm, this would mean that replication of an entire dsDNA molecule would take twice as long. |
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May 7 |
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Are there more descriptive ways of naming genes and gene interactions? This is of only peripheral relevance, but I think that you have got the history of yeast genetic nomenclature backwards. For decades yeast genes were defined functionally and were named accordingly. So, for example, the CDC genes (uppercase = dominant form, usually wild-type, lowercase = recessive form, usually mutant) were described by Lee Hartwell in 1970, about 25 years before the yeast genome was sequenced. At this point ALL of the ORFs/genes were given a systematic name (e.g. CDC1 is YDR182W). The aim is, however, still for all genes to be given a name relating to function as well. |
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May 7 |
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How are zooids identified? There are facts and ruminations at siphonophores.org/SiphOrganization.php From the link it would seem that all zooids in a single colony (animal) are genetically identical, having developed by division from a single zooid. |
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Apr 30 |
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BLAST DNA Sequences Reversed Just to help you with the answers below - there are lots of online tools which allow you to generate the reverse complement of a sequence if you have difficulty 'seeing' this, e.g. bioinformatics.org/sms/rev_comp.html |
