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Sep
5
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Sep
4
comment Literature about putative epigenetic state changes in mammal sequences after cloning steps in Escherichia coli
This is mostly true, but in principle I would like to include any possible bookmarking event.
Sep
4
comment Literature about putative epigenetic state changes in mammal sequences after cloning steps in Escherichia coli
I am interested in finding any record of epigenetic changes while cloning mammal DNA via E. coli (so, the initial DNA would have its epigenetic state, the purified plasmidic DNA after the molecular cloning would have another, possibly different, epigenetic state). By the way, any paper suggesting conservation in the epigenetic state through the cloning steps is also welcome, as it would provide evidence of the lack of changes.
Sep
3
asked Literature about putative epigenetic state changes in mammal sequences after cloning steps in Escherichia coli