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location London, United Kingdom
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visits member for 2 years
seen Aug 30 at 20:08

Java and iOS developer. PhD student in Synthetic Biology.


Aug
30
awarded  Nice Question
Aug
30
awarded  Yearling
Sep
8
comment Why is it sometimes difficult to resuspend E. coli in P1?
@carandraug I also use those. I leave the caps loose and I notice that sometimes the shaker causes them to screw on quite tightly. Even though they seem to grow well (the culture is very cloudy and I get a decent size pellet) I was wondering whether it made them switch completely to anaerobic respiration and cause this gummy pellet somehow. Just a thought.
Sep
6
comment Why is it sometimes difficult to resuspend E. coli in P1?
I'd have to measure next time. What would you consider excessive?
Sep
6
awarded  Supporter
Sep
6
comment Why is it sometimes difficult to resuspend E. coli in P1?
I don't think that the pellets are darker, but I've noticed that sometimes the shaker causes my tubes to screw themselves. Could it be because they don't get enough air?
Sep
6
comment Why is it sometimes difficult to resuspend E. coli in P1?
Yes, I do that too. In the situations I'm talking about, rather than slowly breaking up into a cloud, the pellet will unstick as a whole from the bottom of the tube and clog the tip.
Sep
5
awarded  Student
Sep
5
comment Why is it sometimes difficult to resuspend E. coli in P1?
Straight from 16h culture.
Sep
5
asked Why is it sometimes difficult to resuspend E. coli in P1?
Sep
5
revised Difference between genetic engineering and synthetic biology
added 47 characters in body
Sep
5
awarded  Editor
Sep
5
revised Difference between genetic engineering and synthetic biology
added 47 characters in body
Sep
4
awarded  Teacher
Sep
4
answered Difference between genetic engineering and synthetic biology