meta user
network profile
| bio | website | walkytalky.net |
|---|---|---|
| location | London, United Kingdom | |
| age | 46 | |
| visits | member for | 1 year, 5 months |
| seen | Mar 19 at 15:01 | |
| stats | profile views | 7 |
Erstwhile programmer turned experimental scientist. Now spend more time fiddling with cells and microscopes than coding, but still occasionally keep a hand in.
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Dec 19 |
suggested | suggested edit on |
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Dec 19 |
suggested | suggested edit on |
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Dec 19 |
wiki | |
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Dec 19 |
wiki | |
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Dec 19 |
comment |
How can I keep HEK cells alive while expressing NMDA receptors? It is indeed using a CMV promoter, so that would be something to look at. One suggestion I've seen is to co-transfect with a high ratio of an "empty" pcDNA plasmid as a competitor to reduce expression. That sounds a bit Heath Robinson, but it would be an easier test than splicing into a new vector... |
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Dec 19 |
asked | How is duration of efficacy estimated for vaccines? |
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Dec 19 |
awarded | Quorum |
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Dec 17 |
asked | Are cerebellar basket and stellate neurons actually different cell types? |
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Dec 17 |
revised |
Recommended applications for commercial antibodies removed "immunology" tag added by mdq -- I don't think it is appropriate here, though may have to debate it on meta... |
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Dec 17 |
asked | Recommended applications for commercial antibodies |
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Dec 16 |
awarded | Student |
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Dec 16 |
asked | How can I keep HEK cells alive while expressing NMDA receptors? |
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Dec 16 |
awarded | Precognitive |
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Dec 16 |
awarded | Supporter |
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Dec 16 |
awarded | Autobiographer |
