483 reputation
511
bio website walkytalky.net
location London, United Kingdom
age 47
visits member for 2 years, 11 months
seen Feb 24 at 12:48
Erstwhile programmer turned experimental scientist. Now spend more time fiddling with cells and microscopes than coding, but still occasionally keep a hand in.

Dec
19
wiki created cell-culture description
Dec
19
wiki created cell-culture excerpt
Dec
19
suggested suggested edit on
Dec
19
suggested suggested edit on
Dec
19
wiki
Dec
19
wiki
Dec
19
comment How can I keep HEK cells alive while expressing NMDA receptors?
It is indeed using a CMV promoter, so that would be something to look at. One suggestion I've seen is to co-transfect with a high ratio of an "empty" pcDNA plasmid as a competitor to reduce expression. That sounds a bit Heath Robinson, but it would be an easier test than splicing into a new vector...
Dec
19
asked How is duration of efficacy estimated for vaccines?
Dec
19
awarded  Quorum
Dec
17
asked Are cerebellar basket and stellate neurons actually different cell types?
Dec
17
revised Recommended applications for commercial antibodies
removed "immunology" tag added by mdq -- I don't think it is appropriate here, though may have to debate it on meta...
Dec
17
asked Recommended applications for commercial antibodies
Dec
16
awarded  Student
Dec
16
asked How can I keep HEK cells alive while expressing NMDA receptors?
Dec
16
awarded  Precognitive
Dec
16
awarded  Supporter
Dec
16
awarded  Autobiographer