483 reputation
511
bio website walkytalky.net
location London, United Kingdom
age 47
visits member for 3 years
seen Feb 24 at 12:48
Erstwhile programmer turned experimental scientist. Now spend more time fiddling with cells and microscopes than coding, but still occasionally keep a hand in.

Jan
2
comment Is “exhaustion” of the Hodgkin-Huxley membrane at constant stimulation a real phenomenon?
@Tim For that experiment, I broadly stand by my guess. With sufficient current injection you could overwhelm the oscillatory behaviours of the channel population. It would, however, be interesting to see how closely the real current needed for that matched the model prediction.
Jan
2
comment Is “exhaustion” of the Hodgkin-Huxley membrane at constant stimulation a real phenomenon?
@Tim In which case you probably need to be more specific about the experiment you're proposing. Your question seems to boil down to how fully the H-H model describes the behaviour of an actual axon membrane propagating APs. (There's an auxiliary issue of how well your sim implements the model, but let's shelve that for now.) Not having done the experiment, I can only guess. My intuition is that it would be possible to drive a real membrane to steady state if you're sufficiently aggressive about it, but that doing so would not demonstrate anything meaningful.
Dec
19
comment How can I keep HEK cells alive while expressing NMDA receptors?
It is indeed using a CMV promoter, so that would be something to look at. One suggestion I've seen is to co-transfect with a high ratio of an "empty" pcDNA plasmid as a competitor to reduce expression. That sounds a bit Heath Robinson, but it would be an easier test than splicing into a new vector...