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  • 10 votes cast
Oct
19
asked Genotype-Phenotype databases?
Sep
17
comment What would be the estimated cost sequencing?
Thanks Ankur - yes. These are a family of polymerases with 4 proteins. We are planning to make a rationally designed library of 1000 polymerase variants. 4 plasmids per sample, each of a different sequence, 1000 samples in total.
Sep
17
comment What would be the estimated cost sequencing?
@MattDMo: Yes, that's the plan. It's 4 plasmids per sample, 1000 samples in total, each needs to have (at minimum) its 2 kb insert sequenced. But if we could get full coverage of the plasmid that's cool too.
Sep
17
asked What would be the estimated cost sequencing?
Aug
25
awarded  Autobiographer
Apr
27
comment Ribosomal DNA references sequences
Yes, I have tried the usual sources, my biggest question was whether they had a "reference" genomic 18S rDNA sequence, or if the NCBI sequences themselves were reference sequences.
Apr
27
accepted Ribosomal DNA references sequences
Apr
27
asked Ribosomal DNA references sequences
Mar
6
awarded  Tumbleweed
Dec
26
accepted Clustal Omega - convert from distance into # of substitutions
Apr
23
comment How to determine whether the energy of a stem-loop fold is significant or not?
@caseyr547: I am working on a substring of mRNA that is thought to fold independently of the other substring, because it is not complexed with other proteins.
Apr
23
awarded  Commentator
Apr
23
revised How to determine whether the energy of a stem-loop fold is significant or not?
clarified what kind of stem loop i was referring to.
Apr
23
comment How to determine whether the energy of a stem-loop fold is significant or not?
Yes, it is an RNA. Allow me to update the original question. Many apologies for being unclear.
Apr
22
asked How to determine whether the energy of a stem-loop fold is significant or not?
Mar
25
comment Clustal Omega - convert from distance into # of substitutions
Haha, it looks like I will have to do that in the end. I was trying to see if I could take the lazy route and not write a few lines of code. Thank you for all of your input nonetheless!
Mar
24
revised Clustal Omega - convert from distance into # of substitutions
included how many sequences i am working with
Mar
24
comment Clustal Omega - convert from distance into # of substitutions
Sure, let me update the original question.
Mar
24
asked Clustal Omega - convert from distance into # of substitutions
Mar
21
comment Are there tools for automatically parsing glycan names into tree structures?
No problem, I can brush up on my Java (it's been 6 years since I last used it). Thank you very much!