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Nov
2
awarded  Popular Question
Oct
22
awarded  Teacher
Oct
22
accepted What is the best way to express two proteins in a mammalian cell?
Oct
22
answered What is the best way to express two proteins in a mammalian cell?
Oct
22
revised What is the best way to express two proteins in a mammalian cell?
deleted 18 characters in body
Oct
14
accepted C-myc/anti-myc Ab Interaction with Fusion Proteins
Oct
12
answered C-myc/anti-myc Ab Interaction with Fusion Proteins
Oct
12
revised C-myc/anti-myc Ab Interaction with Fusion Proteins
added 3 characters in body; edited title
Oct
12
asked C-myc/anti-myc Ab Interaction with Fusion Proteins
Sep
23
awarded  Popular Question
Aug
8
awarded  Autobiographer
Mar
18
awarded  Popular Question
Jul
2
awarded  Curious
Dec
13
awarded  Yearling
Apr
15
revised Tool for nucleotide alignment with all nucleotide codes (e.g. R, Y, W, S, etc.)?
added a resource
Apr
15
asked Tool for nucleotide alignment with all nucleotide codes (e.g. R, Y, W, S, etc.)?
Apr
10
awarded  Commentator
Apr
10
comment What is the translation termination efficiency in mammalian cells?
This is a synthetic gene. I already designed a primer with 3 stop codons at the end of the gene. I will post here again if I see any improvement in gene expression (with compared to other similar constructs in our lab).
Apr
10
comment Where to put the gene after eukaryotic promoter for best expression levels?
Thank you all for your comments. I did look to other common constructs and decided to leave around 40 bp between promoter and the initiation codon (this was the minimum distance I could leave because of the restriction site limitation). @Bitwise, I am using mammalian cells (specifically; fibroblast and MDCK cells).
Apr
9
asked What is the translation termination efficiency in mammalian cells?