meta user
network profile
| bio | website | |
|---|---|---|
| location | Montreal, Canada | |
| age | 26 | |
| visits | member for | 1 year, 5 months |
| seen | May 3 at 0:53 | |
| stats | profile views | 22 |
Current Interests
- Directed evolution of proteins
- Protein structure prediction
- Enzymatic chemical synthesis
Past Experience
- Work with sequencing technologies
- Undergraduate-level biochemistry and biology lab work
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Dec 20 |
awarded | Yearling |
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Aug 16 |
comment |
Optimization of annealing temperature I've heard of people using single stand binding protein to reduce primer dimers. |
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Aug 14 |
answered | Why aren't mitochondria and plastids considered symbiotes of eukaryotic cells? |
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Aug 4 |
comment |
How straightforward is in vitro compartmentalization? Thanks for pointing that out, it'll save me some reading, too. Sequencing technologies like 454, SOLiD, IonTorrent use emulsion PCR on a large scale. The SOLiD system even has an sample prep machine that automates the creation of these emulsions. I think these emulsions end up a bit smaller, but they're at least in the right ballpark. |
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Aug 3 |
awarded | Tag Editor |
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Aug 3 |
revised |
added 29 characters in body |
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Aug 3 |
wiki | |
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Aug 3 |
suggested | suggested edit on |
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Aug 3 |
comment |
How straightforward is in vitro compartmentalization? You might want to look up synthesis techniques artificial cells, like artificial red blood cells. A resource that has led me to believe this stuff isn't impossible to set up is at www.artcell.mcgill.ca |
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May 29 |
awarded | Nice Question |
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Mar 28 |
revised |
Do men have more extreme variations than women? update with a slightly more direct answer to the qustion |
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Mar 27 |
comment |
Do men have more extreme variations than women? @Marta, I had put this as a comment, but I switched it to an answer because if I had left it as a comment, it would really be saying that I'm interested in moving to close the question as off-topic. I think it's not enough off topic to close. Besides, if the questioner doesn't know enough to know that the scope of their question is too narrow, a strict interpretation of the rules forcee all feasible answers into the comments section. I am reasonably certain that, as asked, this question has no good answer. |
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Mar 27 |
answered | Do men have more extreme variations than women? |
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Mar 11 |
comment |
Do white Australians have a distinct look? Do you know about alllooksame.com? |
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Mar 4 |
comment |
Why does cyanide inhibit CuZnSOD, but not MnSOD or FeSOD? This answer is not yet a biochemical mechanism. |
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Feb 28 |
answered | Can any protein be phosphorylated? |
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Feb 24 |
comment |
PDB Mining: Why Do I Find Atoms Less than 1 Angstrom Apart? Try looking at the structure in question using a program like Chimera or PyMol, so that you can see how these programs interpret the direction in which the hydrogen should be pointing. |
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Feb 24 |
comment |
Determining potential protease sites within a recombinant protein Are you using E. coli for expression? Using a protease-deficient strain (eg BL21DE3) is usually a good idea for testing this stuff. If you still have truncations, then an analysis of the DNA sequence is in order. |
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Feb 23 |
comment |
Can DNA produce ribozyme-like structures? @bobthejoe It's not a ribozyme, but it does demonstrate a parallel flexibility of conformation and function to ribozymes. Part of the question addresses whether there is a biochemical reason "stopping the folding". |
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Feb 21 |
answered | Can DNA produce ribozyme-like structures? |
