1,214 reputation
414
bio website
location Montreal, Canada
age 27
visits member for 2 years, 7 months
seen Jul 8 at 16:18

Current Interests

  • Directed evolution of proteins
  • Protein structure prediction
  • Enzymatic chemical synthesis

Past Experience

  • Work with sequencing technologies
  • Undergraduate-level biochemistry and biology lab work

Jun
23
comment Are there critical points in ecosystems?
Another (overlapping) interesting place to look: the concept of keystone species.
Jan
14
comment Could murder be modeled as an infectious disease?
@Fomite Good point. Also, nice answer.
Dec
20
awarded  Yearling
Dec
15
comment How easy is it to carry out de novo sequence assembly?
"If you only want to use only sequencing techniques, you have a problem." -- Do you mean resequencing techniques, or high-throughput sequencing techniques?
Dec
15
comment How easy is it to carry out de novo sequence assembly?
De novo sequencing without using some long-read techniques for cleanup (like Sanger sequencing) requires a lot of coverage, and you still end up with substantial uncertainty in the results, due to the large amount of repetitive sequences that are longer than 30bp in most genomes. The reply by @bitwise gives a great idea of what I was thinking.
Dec
9
comment How easy is it to carry out de novo sequence assembly?
would it really be necessary to use 30bp reads? that's very short.
Dec
6
revised What is meant in biology by the term “evolved”?
added 248 characters in body
Dec
6
answered Bacterial cell lysis buffer used in proteomics procedures
Dec
6
revised Bacterial cell lysis buffer used in proteomics procedures
removed "thank you", created complete sentences
Dec
6
comment Could murder be modeled as an infectious disease?
While it's probably possible to create an epidemiological model that mimics the spread of murders, it would be ethically dangerous territory to study in a controlled way to get a dataset that points to causes (and not correlations).
Dec
6
comment Chemoautotrophy in large organisms?
@satwik-pasani While they would still probably be considered heterotrophs, there are a variety of insects that spit gastric(-like) juices onto prey and externally digest them, then suck up what's left.
Dec
6
comment Chemoautotrophy in large organisms?
@shigeta There's also an advantage to ingesting and internally digesting biotic material - once you've eaten, you're no longer in competition with your neighbors those nutrients, so your meal can't be cut short by crowding.
Dec
6
answered Is there a limit to acceptable brightness for plants?
Dec
6
revised Is there a limit to acceptable brightness for plants?
edits for clarity, grammar
Dec
20
awarded  Yearling
Aug
16
comment Optimization of annealing temperature
I've heard of people using single stand binding protein to reduce primer dimers.
Aug
14
answered Why aren't mitochondria and plastids considered symbiotes of eukaryotic cells?
Aug
4
comment How straightforward is in vitro compartmentalization?
Thanks for pointing that out, it'll save me some reading, too. Sequencing technologies like 454, SOLiD, IonTorrent use emulsion PCR on a large scale. The SOLiD system even has an sample prep machine that automates the creation of these emulsions. I think these emulsions end up a bit smaller, but they're at least in the right ballpark.
Aug
3
awarded  Tag Editor
Aug
3
revised
added 29 characters in body