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Dec
3
comment How to find miRNA binding sites on a specific gene?
Bioinformatic part of your answer may be better that is why i did not give full credit. For those who also would like to know more about tools for finding miRNA target site they could check this article. It is really good: journal.frontiersin.org/Journal/10.3389/fgene.2014.00023/full Thanks for reminding me of the question by the way.
Oct
26
comment How to find miRNA binding sites on a specific gene?
yes "For the time being i am just looking for a good candidate list of miRNAs for a specific gene (the gene of my choice)". So, i want to choose a specific gene in the database and i want that database to show me a list of mirna's that can bind to that gene's 3'UTR . Also, I want to see the accountability of mirna binding via a scoring method such as mirSVR. I have so far found only mirSVR and the mentioned website but are there other ways and what is the best way to search for that in the literature? sorry if i am not maintaining correct terminology, i am relatively new in this.
Oct
26
comment How to find miRNA binding sites on a specific gene?
Thank you for the answer but my question was actually a lot more simpler. For the time being i am just looking for a good candidate list of miRNAs. I have found a website (microrna.org/microrna/home.do) for this and it is based on mirSVR score. Is this a good way of picking candidate miRNA's? Are there other ways of finding candidate miRNA's that are based on different scoring algorithm?
Jun
18
comment what is the difference between “cistron” and “gene”
off the top of my head: cistron and gene are equivalent to each other but cistron is a bit old-fashioned. Consider two phenotypes in different individuals belonging to certain organism; let's say both of them are recessive and you cross these phenotypes with one another. If you see wild type phenotype in the next offspring then it means your phenotypes reside in different regions in the genome and these regions were first defined as cistrons. Of course it then turned out to be much more complicated than that and people started using the term gene more often
Jun
13
comment What is Pan for in pan-caspase?
there is no specific context but i now realized that it is usually used as pan-caspase inhibitor in the articles i was looking at. just to be sure: can we then say that pan is the opposite of specific?